小鼠大脑中动脉栓塞模型脑血流量与梗死体积相关性分析

Correlation between cerebral blood flow and infarct volume of the middle cerebral artery occlusion in mice

目的:探讨线栓法制备小鼠局灶性脑缺血再灌注模型成功的两种评价方法间的相关性,为评价该模型提供一种新的方法和标准。方法:20只雄性KM小鼠,随机分为模型组(n=10)、假手术组(n=10),采用线栓法阻塞大脑中动脉(MCA)建立局灶性脑缺血再灌注模型,分别于术前、缺血即刻、缺血2 h、再灌注即刻、再灌注24 h利用激光散斑成像技术监测各组小鼠脑血流量变化,再灌注24 h后进行神经功能评分,并行TTC染色法检测脑梗死体积百分比,相关分析判断脑血流量变化与梗死体积百分比之间的相关性。结果:与假手术组相比,模型组小鼠出现明显的神经功能异常,TTC染色显示出明显的脑梗死,梗死侧脑血流量明显降低,差异有统计学意义(P<0.05)。缺血即刻、缺血2 h小鼠脑血流量变化与再灌注24 h脑梗死体积的相关系数R2值分别为0.55、0.28(P<0.05)。结论:缺血即刻的皮层血流变化与梗死体积具有较好的线性相关。小鼠脑血流量变化是除神经功能评分和脑梗死体积以外另一种可判断小鼠局灶性脑缺血模型制作成功的可靠且实用的方法。

AIM： To analyze the correlation be- tween the two evaluation methods of middle cerebral artery occlusion and focal cerebral ischemia so as to explore a novel method and standard for the evalua- tion of the model. METHODS： Twenty adult male kunming mice were randomly divided into the model group and the sham-operated group（n = 10）. The fo- cal cerebral ischemia reperfusion model was estab- lished by thread embolism method, and the changes of cerebral blood flow in each group were detected beforeischemia, the moment of ischemia, two hours after ischemia, immediately and 24 h after reperfu- sion. The rating system was employed to evaluate neurological function of mice at 24 h after ischemia reperfusion, the brain infract volume percentage was assessed after 2% TI＇C staining following the last neurological evaluation, and the correlation between the infarct volume and cerebral blood flow ratio was analyzed by correlation analysis. RESULTS：Cornpared with sham group, the mice in the model group exhibited obvious neurological abnormalities, the Trc staining showed obvious infarction, and the cer- ebral blood flow of the infarct side was significantly decreased （ P 〈 0.05 ）. The correlation coefficient of the cerebral blood flow and the infarct volume at 24 h after reperfusion were 0.55 and 0.28 （ P 〈 0.05 ） , respectively. CONCLUSION： There was a high linear correlation between cerebral infarct volume at 24 h reperfusion and cerebral blood flow immediately after ischemia in model group. Cerebral blood flow is a reliable indicator to confirm the establishment of mouse MCAO model besides monitoring theneurolog- ical function evaluation and infarct volume