Caveolin-1在子宫内膜癌组织中的表达及临床意义

Clinical significance of Caveolin-1 expression in endometrial carcinoma

目的探讨窖蛋白-1(Caveolin-1)在子宫内膜癌组织中的表达及其与临床病理特征的关系。方法采用免疫组化法及蛋白质印迹法检测45例子宫内膜癌组织(内膜癌组)、35例子宫内膜良性病变组织(良性组)和35例正常子宫内膜组织(正常组)中Caveolin-1蛋白的表达。实时荧光定量PCR法检测内膜癌组中Caveolin-1 m RNA的表达,并分析其与临床病理特征的关系。结果 Caveolin-1蛋白在内膜癌组、良性组、正常组中的表达呈递减趋势,差异有统计学意义(P<0.05);在内膜癌组淋巴结转移者Caveolin-1 m RNA表达高于无淋巴结转移者(P=0.001);TNM分期Ⅱ期、Ⅲ+Ⅳ期者高于Ⅰ期者(P=0.002,P=0.040);低分化、中分化者显著高于高分化者(P<0.001,P=0.020);Caveolin-1在子宫内膜癌组织中的表达与年龄及组织分型无明显相关性(P>0.05)。结论 Caveolin-1在子宫内膜癌组织中高表达,且与淋巴结转移、分化程度和TNM分期有关,有望成为子宫内膜癌早期诊断的指标及治疗靶点。

Objective To evaluate the expression of Caveolin-1 in endometrial carcinoma and investigate its association with pathological features. Methods Caveolin-1 expression was determined by immunohistochemistry and Western blot using rabbit polyclonal antibody in 45 cases of endometrial carcinoma,35 cases of hyperplastic endometrium,and 35 cases of normal endometrium.Caveolin-1 mRNA levels in endometrial carcinoma tissue were determined using real-time PCR. Results Immunohistochemistry and Western blot showed different rates of positive expression of Caveolin-1 in the different types of endometrium. Caveolin-1 expression tended to increase with degree of malignancy （P〈0.05）.Among cases with endometrial carcinoma, Caveolin-1 expression was much higher in patients with lymph node metastasis （ P= 0. 001） , and it was higher in stages Ⅱ and Ⅲ＋Ⅳ than in stage （P=0.002,P=0.040）. Caveolin-1 expression was much higher in moderate and low-grade endometrial carcinoma than in high-grade endometrial carcinoma （P〈0.001, P=0.020 ）. Conclusions Caveolin-1 expression is higher in endometrial carcinoma than in hyperplastic or normal endometrium,and it correlates significantly with tumor stage and lymph node metastasis.Caveolin-1 may play an important role in endometrial carcinoma progression.