体外操作对人卵母细胞线粒体膜电位影响的研究

Effect of in vitro manipulation on mitochondrial membrane potential of human oocytes

目的比较体外不同操作对人卵母细胞线粒体膜电位的影响。方法利用JC-1试剂盒检测人卵母细胞在体外成熟培养、玻璃化冷冻解冻、卵胞质内单精子注射(ICSI)前后的线粒体膜电位变化,从而得出体外不同操作对线粒体膜电位的影响及卵母细胞质量与线粒体膜电位的关系。结果卵母细胞在体外正常培养成熟、成熟卵母细胞玻璃化冷冻解冻及卵母细胞显微操作过程中线粒体膜电位均无统计学变化(P>0.05)。体外成熟培养失败及单精子注射后受精失败的卵母细胞线粒体膜电位有明显下降(P<0.05)。结论体外常规操作对卵母细胞线粒体膜电位并无显著影响,但是线粒体膜电位与卵母细胞质量有密切关系,可以作为检验卵母细胞质量的一个重要指标。

Objective To compare the effects of different operations on the mitochondrial membrane potential of oocytes in vitro. Methods The mitochondrial membrane potential of oocytes, treated by oocyte maturation in vitro, vitrification and intracytoplasmic sperm injection （ICSI）, were detecdted by JC-1 kit. Results No significant changes were observed in the mitochondrial membrane potential during the oocytes maturation in vitro, vitrification of oocytes and the micromanipulation of oocytes. At the same time, there was a significant decrease in the mitochondrial membrane potential of oocytes after the failure of in vitro maturation, and the failure of fertilization after single sperm injection.Conclusion In vitro routine operation has no obvious effect on the mitochondrial membrane potential of oocyte, but there is a close relationship between mitochondrial membrane potential and oocyte quality. Therefore, mitochondrial membrane potential can be used as a new indicator to evaluate the quality and a supplement to the morphological evaluation.