葛根素对大鼠心肌缺血再灌注损伤的影响及作用机制探讨

Puerarin on myocardial ischemia-reperfusion injury in rats and the influence of the mechanism of action

目的旨在探讨葛根素对心肌缺血再灌注损伤(MIRI)的影响及作用机制。方法选择结扎大鼠左前降支动脉形成急性心肌梗死+再灌注3 h,成功建立大鼠MIRI模型后分为模型组和葛根素组,每组大鼠各20只。再灌注12 h后,测定和比较两组大鼠心肌梗死面积及心肌细胞凋亡指数,免疫组化法测定和比较两组大鼠心肌组织Bcl-2、Bax蛋白表达。结果葛根素组大鼠心肌梗死面积(20.21±6.85)%显著低于模型组大鼠(47.19±8.93)%(P<0.01),葛根素组大鼠心肌细胞凋亡指数(15.19±1.55)%显著低于模型组大鼠(21.46±2.43)%(P<0.01)。葛根素组Bax蛋白表达显著低于模型组大鼠(P<0.05),葛根素组Bcl-2蛋白表达显著高于模型组大鼠(P<0.05)。结论葛根素可降低MIRI引起心肌损伤程度,其作用机制可能和调节血清相关促细胞凋亡基因蛋白及信号通路功能相关。

Objective：To investigate the effect of Puerarin on myocardial ischemia reperfusion injury （MIRI） and its mechanism. Methods：Rats were divided into model group and Puerarin group, with 20 rats in each group when the MIRI models were successfully established. 12h after repcrfusion, serum superoxide dismutase determination and comparison of two groups of rats （SOD） activity, malondialdehyde （MDA）, lactate dehydrogenase （LDH） and glutathione peroxidase （GSH-Px） levels were measured and compared in two groups. Areas of myocardial infarction and myocardial cell apoptosis index of two groups were measured and compared with. The expression levels of Bcl-2 and Bax protein were determined by immunohisto- chemistry and compared between the two groups of rat myocardial tissue, and the expression of Caspase-3, Akt and p-Akt were measured by Western blot method and compared between the two groups in myocardial tissue of rats. Results ： Puerarin group myocardial infarct size in rats （20.21 ± 6.85 ）% was significantly lower than that of model group rats （47.19 ± 8.93 ）% （P 〈 0. 01 ）, and Puerarin group myocardial apoptosis index （ 15.19 ± 1.55） % was significantly lower than that of model group rats （21.46 ± 2.43） % （ P 〈 0. 01 ）. The expressions of SOD and GSH-Px in the puerarin group were significantly higher than those in the model group （P 〈 0. 05 ）, and the expressions of LDH and MDA in the puerarin group were significantly lower than those in the model group （P 〈 0.05 ）. The expression of Bax protein in Puerarin group was significantly lower than that in model group （P 〈 0. 05）, and the expression of Bcl-2 protein in Puerarin group was significantly higher than that in model group （P 〈 0. 05）. The expression of Caspase-3 protein in Puerarin group was significantly lower than that of the rats in the model group （P 〈 0. 05）, there were no significant differences between Akt protein and the rats in the model group （P 〉 0. 05 ）, the expressions of p-Akt protein and p-Akt/Akt were significantly higher than those of the rats in the model group （P 〈 0. 01 ）. Conclusion： Puerarin can decrease the degree of myocardial injury induced by MIRI, and its mechanism may be related to the regulation of serum SOD, MDA and LDH expression, GSH-Px activity and related Pro apoptotic gene protein and signal pathway function.