通过转录组学探讨外膜蛋白相关基因在多药耐药鲍曼不动杆菌生物被膜中的异常调控

Transcriptional study of outer membrane protein in abnormal regulation about biofilm

目的通过研究转录组学探讨外膜蛋白相关基因在多药耐药鲍曼不动杆菌生物被膜生成中的作用。方法收集筛选我院多药耐药鲍曼不动杆菌菌株。高通量测序分析鲍曼不动杆菌在不同的生长状态下基因表达的差异。结果多药耐药鲍曼不动杆菌生物被膜状态与快速生长期相比，确实存在基因表达差异。表达差异的基因中，106个基因表达上调，92个基因表达下调。其中，外膜蛋白的相关基因在生物被膜状态是显著表达的。结论细菌的生物被膜状态是一种独特的存在形式。在生物被膜状态下，表达差异的基因集中于菌体外膜蛋白的表达和细胞内通路的因子调控。外膜蛋白是鲍曼不动杆菌生物被膜生成过程中表达最丰富的蛋白之一，在菌体生物被膜形成和成熟过程中发挥重要作用。

Objective To study the role of outer membrane protein in the formation of multidrug resistant Acinetobacter baumannii biofilm. Methods The multidrug resistant strains of Acinetobacter baumannii in our hospital were selected. The same strain was studied in different growth states, the whole genome of the two samples was compared. The gene function was analyzed by high-throughput sequencing. Statistical differences in gene expression between two samples were studied. Results The gene expression of the same strain in two different states was indeed different. Among the differentially expressed genes, 106 genes were up-regulated and 92 genes were down-regulated. Among them, the outer membrane protein related genes in the biofilm state were significantly expressed. Conclusions The biofilm was a unique form. In the biofilm state, the different genes focus on the expression of the outer membrane proteins and the regulation of intracellular pathways. Outer membrane protein, which is one of most abundant proteins in the biofilm formation, plays an important role in the process of biofilm formation and maturation.