仔猪黄痢病原菌分离鉴定与中西医结合治疗实验

Isolation and identification of piglets yellow dysentery pathogens and study on its treatment by traditional Chinese medicine combined with western medicine

通过病猪病原菌的分离鉴定,对病例进行确诊,检测分离菌的耐药性,探讨中西医结合治疗仔猪黄痢的方法。采用细菌分离培养、显微镜下形态观察、生化试验、细菌毒力试验等方法,对旧城镇不同地区3个规模化养猪场的3起疑似仔猪黄痢病例进行了病原菌的分离鉴定,同时对分离菌进行纸片法常规药敏试验。结果显示,3起病例中均分离到了致病性大肠杆菌;对病原菌高敏药物分别是恩诺沙星、环丙沙星;中西医结合治疗有效率达96.1%,死亡率为3.9%;西药组有效率为82.0%,死亡率为18.0%;对照组死亡率高达87.9%。说明中西医结合治疗优于单纯使用西药治疗,两者差异显著(P<0.05)。

In this study, pathogenic bacteria were isolated and identified from three suspected piglets of three pig farms in different areas of the old town by bacterial isolation and culture, microscopic observation, biochemical tests and bacterial toxicity test, at the same time, carrying out conventional drug susceptibility test paper method. Results showed that three cases were isolated pathogenic E.coli. The high sensitivity drugs were enrofloxacin and ciprofloxacin. Treatment efficiency was 96.1% by the combination of traditional Chinese medicine with western medicine, and the mortality rate was 3.9%; Effective rate in western medicine group was 82.0% with the death rate of 18.0%： The mortality rates in control group was as high as 87.9%. Combined therapy of traditional Chinese medicine with western medicine was better （P〈0.05） than using western medicine alone.