监测动脉血压的清醒重症中暑大鼠模型的构建

The establishment of heat stroke model of conscious rats monitored with arterial blood pressure

目的:探索监测动脉血压的清醒大鼠重症中暑模型的构建方法。方法:随机将40只雄性大鼠分为清醒中暑组、麻醉中暑组、清醒对照组和麻醉对照组。其中,清醒中暑组的大鼠在腹腔注射麻醉情况下完成右侧颈总动脉置管并固定,待其清醒后再置于高温高湿环境接受热打击,直至其体温超过42℃及血压下降;麻醉中暑组在动脉置管后保持大鼠在麻醉状态接受热打击;清醒、麻醉对照组则置于常温常湿环境中。比较各组生命体征、热负荷量、体重丢失、生存率指标的变化。结果:较麻醉中暑组而言,清醒对照组具有显著增加的热负荷量及体重丢失,其生命体征变化更平缓、生存率也更高。结论:监测动脉血压的清醒大鼠模型更加符合重症中暑致病过程中的病理生理学特点,更能满足后续动物实验的需要。

Objective：To explore the method of establishing heat stroke model of conscious rats monitored with arterial blood pressure. Method：Forty male rats were randomly and equally divided into four groups, including con scious heatstroke group, unconscious heatstroke group, conscious control group and unconscious control group. Catheterizations were proceeded on the right common carotid artery and fixed in the conscious heatstroke rats un- der intraperitoneal anesthesia. They would not put into hot and humid artificial climate environment until the con- sciousness were regained. High core temperature exceeding 42℃ and drop of blood pressure were observed when heat stress were closured. The rats in the unconscious heatstroke group receiving carotid artery catheterizations and intraperitoneal anesthesia were exposed to heat stress under continuous anesthesia. Rats of the conscious and un- conscious control group were observed in environment with normal temperature and humidity. Indicators of heat stress responds including vital signs, thermal load, loss of weight and survival time were compared between groups. Result： Comparing with unconscious heatstroke group, increasing thermal load and loss of weight, more gentle change of vital sign and better survival were found out in the conscious heatstroke group. Conclusion：The model of conscious rats monitored with arterial blood pressure were more suitable in the respect of pathophysiology charac teristic during the pathogenic process of heat stroke,which might better satisfy further animal experiments.