蒙古扁桃编码Ca^(2+)结合蛋白基因的系统性鉴定和表达分析

Mongolian Almond Ca^(2+) Binding Protein Encoding Gene Systemic Identification and Expression Analysis

为进一步解析蒙古扁桃抗逆境的遗传机理,采用高通量转录组测序技术,结合生物信息学和基因表达分析,在蒙古扁桃体内鉴定到16个编码Ca^(2+)结合蛋白的全长转录本,包括4个Ca^(2+)ATPase(ECA/ACA),3个具有多个跨膜域的新型Ca^(2+)通道蛋白(ERD),5个Ca^(2+)/H+反向转运蛋白(CAX),2个Ca^(2+)依赖的蛋白激酶(CPK)和2个钙调素蛋白(CAM),它们的结构域同拟南芥同源蛋白基本相同。ERD、CAX、CPK和CAM各有1个基因在蒙古扁桃根和叶中有明显表达,其中的ERD、CPK和CAM类的基因表达在不同程度上受干旱和盐胁迫调节,说明有可能调节蒙古扁桃的抗逆能力。

Mongolian Almond（Prunus mongolica Maxim）belongs to Rosaceae,under national protection of second class,primarily distributes at the Northwestern Dessert Region of China and is tolerant to drought and poor soil.Calcium ion is the most important signaling molecule for plant sensing and resistance to environment stresses.In this study,based on transcriptome analysis of the leaf,we identified sixteen strings of full length transcripts,including four Ca^2＋ ATPase（ECA/ACA）,three novel Ca^2＋ channel protein with several transmembrane domains（ERD）,five Ca^2＋/H＋antiporter（CAX）,two Ca^2＋ dependent protein kinase（CPK）and two calmodulins（CAM）.Their predicated structures are similar to those homologies in Arabidopsis.Four single genes from the ERD,CAX,CPK and CAM categories,respectively,had obvious expression in the leaves and roots,among which the ERD,CPK and CAM genes expression had been regulated by drought and salt at different extent.It indicated that the genes might modulate the plant′s environmental stress-tolerant ability.