复方紫红参多糖对H_(22)荷瘤小鼠的抑瘤作用研究

Study on anti-tumor effects of Compound Purple Ginseng polysaccharides on H_(22) tumor-bearing mice

目的探讨复方紫红参多糖(CPGP)对小鼠肝癌H_(22)实体瘤的抑制作用及机制。方法 ICR雄性小鼠60只,除空白组外,其余小鼠建立H_(22)荷瘤小鼠模型,随机分为模型组、环磷酰胺(CTX)组(25μg/g)和复方紫红参多糖(CPGP)低、中、高剂量组(70、140、280μg/g),每组10只。各组给予相应干预,共10天。观察小鼠一般状况,计算抑瘤率、脏器指数;ELISA法测定血清TNF-α、IL-2、IFN-γ等指标;采用流式细胞术对脾脏组织T淋巴细胞亚群进行测定。结果环磷酰胺(CTX)组和CPGP低、中、高剂量组对肝癌H_(22)的抑瘤率分别为65.01%、40.19%、51.32%、57.84%。与模型组比较,CPGP各组显著提高小鼠体质量、免疫器官指数(P<0.05,P<0.01),CPGP高剂量组能够升高血清中TNF-α、IL-2、IFN-γ(P<0.05),CPGP各组脾T淋巴细胞亚群CD4^+/CD8^+的比值升高(P<0.01);而CTX组小鼠体质量、免疫器官指数,TNF-α、IL-2、IFN-γ水平及脾T淋巴细胞亚群CD4^+/CD8^+的比值均显著降低(P<0.05,P<0.01)。与CTX组比较,CPGP小鼠体质量、免疫器官指数,TNF-α、IL-2、IFN-γ水平及脾T淋巴细胞亚群CD4^+/CD8^+的比值均显著升高(P<0.05,P<0.01)。结论 CPGP对小鼠肝癌H_(22)具有明显的抑制作用,其机制可能与提高机体免疫功能有关。

Objective To study the inhibitory effect and mechanism of Compound Purple Ginseng polysaccharides （CPGP） in hepatocarcinoma 22 （ H22 ） transplanted mice. Methods 60 male ICR mice were selected and H22 tumor-bearing mice model was established except the blank group. Then the mice were randomly divided into the model group ,cyclophosphamide（ CTX ,25 μg/g） , CPGP groups with low-（ 70 μg/g） , middle-（ 140 μg/g）, high-（ 280 μg/g） dose, 10 mice in each group. Each group were given the corresponding intervention, with a course of 10 days. The general condition of mice was observed. The tumor inhibition rate and organ indexes were calculated. The serum levels of TNF-α, IL-2, IFN-γ were determined by ELISA. The T-lymphocyte subsets in spleen were detected by flow cytometry. Results The tumor inhibition rates of CTX group and CPGP groups with low-, middle- and high-dose on H22 were 65.01%, 40.19% ,51.32 % ,57.84% respectively. Compared with the model group, the body weight and immune organ indexes were significantly increased in CPGP groups with low-, middle- and high-dose （ P 〈 0.05, P 〈 0.01 ）, the serum levels of TNF-α, IL-2, IFN-γwere increased in CPGP group with high-dose （ P 〈 0.05 ）, and the ratio of CD4 ＋/CD8＋ in splenic T-lymphocyte subsets was increased in CPGP groups with low-, middle- and high-dose （ P 〈 0.01 ）. Compared with the model group,the body weight and immune organ indexes,the serum levels of TNF-α, IL-2, IFN-3, and the ratio of CD4＋/CD8＋ in splenic T-lymphocyte subsets were significantly decreased in CTX group（ P 〈0.05 ,P 〈 0.01 ）. Compared with CTX group, the body weight and immune organ indexes, the serum levels of TNF-α, IL-2, IFN-γ and the ratio of CD4＋/CD8＋ in splenic T-lymphocyte subsets were significantly increased in CPGP-treated mice （ P 〈 0.05,P 〈 0.01 ）. Conclusion CPGP shows significantly inhibitory effects on transplanted H22 tumor in mice. Its mechanism may be associated with enhancing immune function.