TCF7L2基因rs3814570多态性与新疆维吾尔族人群T2DM的相关性研究

Study on correlation between TCF7L2 gene rs3814570 polymorphisms with T2DM in Uygur Population of Xinjiang

目的探讨新疆地区维吾尔族人群转录因子7类似物2(TCF7L2)基因的rs3814570位点与2型糖尿病(T2DM)的相关性。方法采用病例-对照研究设计,以经确诊的949例T2DM患者作为观察组,选取963例健康体检者作为对照组。应用基质辅助激光解吸附电离飞行时间质谱分析质谱技术(MALDI-TOF)对TCF7L2基因多态性进行检测。结果两组对象rs3814570位点基因型CC、CT和TT,等位基因C和T的分布比较,差异有统计学意义(P<0.05),CT基因型携带者患T2DM的风险是CC基因型携带者的0.331倍(OR=0.331,95%CI:0.166~0.661,P=0.002),TT基因型携带者患T2DM的风险是CC基因型携带者的0.539倍(OR=0.539,95%CI:0.348~0.834,P=0.005),T等位基因携带者患T2DM的风险是C等位基因携带者的0.501倍(OR=0.501,95%CI:0.377~0.664,P<0.01)。两组对象中,TCF7L2基因rs3814570位点的CT+TT基因型组的空腹血糖(FPG)水平均明显低于CC基因型组(P<0.05)。结论 TCF7L2基因的rs3814570位点可能与新疆维吾尔族人群T2DM的发生相关,T等位基因和TT基因型可能是T2DM发生的保护因素。

Objective To investigate the correlation between TCF7L2 gene rs3814570 polymorphisms with type 2 diabetes mellitus（T2DM） in Uygur population of Xinjiang area. Methods By adopting the case-control study design,949 cases of T2DM were recruited as the observation group and 963 individuals undergoing healthy physical examination were selected as the control group. The TCFTL2 gene polymorphism was detected by matrix-assisted laser desorption/ionization-time of flight（MALDI-TOF）. Results The statistical differences in frequencies of CC, CT and TT genotypes and the C and T allele frequencies on TCFTL2 rs3814570 were found between the T2DM group and control group（P〈0.05）. The risk of suffering from T2DM in the carriers of CT genotype was 0.331 times of that in the carriers of CC genotype （OR = 0.331,95 % CI：0. 166- 0.661, P = 0. 002）, the risk of suffering from T2DM in the carriers of TT genotype was 0. 539 times of that in the carriers of CC genotype（OR= 0. 539,95 %CI： 0. 348-0. 834,P〈0. 005） ,and the risk of suffering from T2DM in the carriers of T allele was 0. 501 times of that in the carriers of C allele（OR= 0. 501,95%CI：0. 377-0. 664,P〈0.01）. Among all subjects,the FPG level of the CT＋TT genotype group on TCFTL2 gene rs3814570 locus was significantly lower than that of the CC genotype group（P〈0.05）. Conclusion The rs3814570 locus in TCFTL2 gene may,be associated with T2DM occurrence in Uygur population of Xinjiang area, the T allele and TT genotype might be protective factors of T2DM.