双歧杆菌介导CTP-NPRL2对裸鼠肾癌生长及凋亡的影响

Effect of bifidobacterium-mediated CTP-NPRL2 on growth and apoptosis of nude mouse renal carcinoma

目的探讨双歧杆菌介导CTP-NPRL2对裸鼠肾癌生长及凋亡的影响。方法构建pET15b-CTP-NPRL2并电转双歧杆菌,Western blot验证蛋白表达。采用肾癌细胞悬液皮下注射法建立裸鼠肾癌模型,并分为观察组与对照组(每组8只),分别经尾静脉注射携带pET15b-CTP-NPRL2的双歧杆菌与生理盐水,每周1次,4周后处死裸鼠,称取裸鼠及荷瘤质量,TUNEL法检测肾癌组织凋亡。结果观察组与对照组裸鼠质量分别为(26.24±1.98)g、(23.28±2.17)g,荷瘤质量分别为(1.37±0.12)g、(1.68±0.18)g,两组比较差异均有统计学意义(P<0.05)。TUNEL检测显示观察组凋亡指数(23.27±5.14)%明显高于对照组(10.37±2.58)%,两组比较差异有统计学意义(P<0.05)。结论双歧杆菌介导CTP-NPRL2对裸鼠肾癌生长具有抑制功能,并明显增加了肾癌细胞的凋亡。

Objective To evaluate the effect of bifidobacterium-mediated CTP-NPRL2 on the growth and apoptosis of nude mouse renal carcinoma. Methods Recombinant plasmid pET15h-CTP-NPRL2 was constructed and transfected into bifidobacterium by electroporation,and then the expression of fusion protein CTP-NPRL2 was verified by Western blot. The nude mouse renal carcinoma model was constructed by subcutaneous injection of renal carcinoma cells suspension. The nude mice with renal carcinoma were equally and randomly divided into the observation group and control group（8 mice in each group）. The mice in the observation group were treated with bifidobacterium containing recombinant plasmid pET15b-CTP-NPRL2 through tail vein injection,and the mice in the control group were treated with normal saline. All mice were treated once a week for four weeks,and then executed for evaluating the weight of mice and bearing tumors. Finally, the apoptosis of renal carcinoma was detected by TUNEL staining. Re- sults The mass of nude mice was（26.24±1.98） g in the observation group and（23.28±2.17） g in the control group. The mass of bearing tumors was（1.37±0.12） g in the observation group and（1.68±0.18） g in the control group,and the differences were statistically significant（P〈0.05）. The TUNEL detection results showed that the apoptosis index of renal carcinoma cell in the observation group（23.27±5.14） % was significantly higher than that in the control group（10.37±2.58） %,and the difference was statistically significant（P〈0.05）. Conclusion Bifidobacterium-mediated CTP-NPRL2 has an inhibitory effect on the renal carcinoma growth of nude mouse,and significantly increases the apoptosis of renal carcinoma ceils.