大豆GmMYB130对GmCHI3基因的转录调控研究

Transcriptional Regulation of GmMYB130 on GmCHI3

盐胁迫通常会引起植物细胞内过氧化物等次级危害,是限制农作物产量的主要逆境之一,从分子水平解析大豆在盐胁迫中的响应机制可为大豆耐盐品种的分子育种奠定基础.本研究通过亚细胞定位、染色质免疫共沉淀、荧光定量PCR等手段,探索大豆耐盐响应因子GmMYB130对GmCHI3基因的转录调控机制.结果表明,GmMYB130定位于细胞核中,且能结合于类黄酮合成酶基因GmCHI3的启动子区,从而调节GmCHI3的转录表达.因此,转录因子GmMYB130可能通过激活GmCHI3的转录,调节类黄酮物质的合成,最终提高大豆耐盐能力.

Salt stress, usually brings osmotic stress to plant cells, is one of the most abiotic stresses limiting crop productivities. Understanding of the intracellular responses to salinity will favor the molecular breeding of salt tolerant soybean cultivars. In this study, subcellular localization, ChIP and qRT-PCR assays are used to explore the transcriptional regulation of GrnCHI3 by the salt responsive GmMYB130 protein. The results show that GmMYB130 localizes in the nucleus of soybean roots. Interestingly, GmMYB130 bounds to the promoter region of the GmCHI 3 gene and activates its transcription. Hence, the transcription factor GmMYB130 is supposed to enhance the soybean tolerance via the regulation of flavonoid synthesis.