肺癌患者血清中21种细胞因子的表达水平及其临床意义

Expressions and clinical significance of 21 cytokines in the serum of patients with lung cancer

目的:检测肺癌患者血清中21种细胞因子[干扰素诱导的T细胞趋化因子(IFN-inducible T cellαchemoattractant,ITAC)、GM-CSF、Fractalkine、IFN-γ、IL-10、巨噬细胞炎性蛋白-3α(macrophage inflammatory protein-3α,MIP-3α)]、IL-12(p70)、IL-13、IL-17A、IL-1β、IL-2、IL-4、IL-21、IL-23、IL-5、IL-6、IL-7、IL-8、MIP-1α、MIP-1β和TNF-α的表达情况并分析其意义。方法:收集2015年3月至6月的山西省肿瘤医院40例肺癌患者;采用液相芯片技术检测30名健康人和40例初诊肺癌患者治疗前血清中21种细胞因子的表达水平,分析其与肺癌临床特征之间的关系及表达存在明显差异细胞因子间的相关性。结果:肺癌患者血清中11种细胞因子[(GM-CSF、Fractalkine、IFN-γ、MIP-3α、IL-12(p70)、IL-1β、IL-2、IL-6、IL-7、IL-8、TNF-α]的表达水平明显升高(P<0.05或P<0.01),肺癌患者非转移组与转移组血清中21种细胞因子的表达水平比较无明显差异(P>0.05),肺腺癌(adenocarcinoma,AC)组血清IFN-γ与MIP-1β水平明显高于鳞癌(squamous cell carcinoma,SCC)组(均P<0.05),肺SCC组血清ITAC表达水平明显高于小细胞肺癌(small cell lung cancer,SCLC)组(P<0.05)。高表达的11种细胞因子在两组间表达的相关性不同。结论:肺癌患者血清中IL-6、IL-8等11种细胞因子表达升高,可能参与了肺癌发生发展,这些高表达的细胞因子或许可用于肺癌的辅助诊断,并为肺癌治疗提供新的靶标。

Objective：To detect the expressions of 21 cytokines （ITAC[IFN inducible T cell α chemoattractant], GM CSF, Fractalkine, IFN γ, L 10, MIP 3α [macrophage inflammatory protein 3α], IL 12（p70）, IL 13, IL 17A, IL 1β, IL 2, IL 4, IL 21, IL 23, IL 5, IL 6, IL 7, IL 8, MIP 1α, MIP 1β and TNF α） in serum of patients with lung cancer, and analyze their clinical significance. Methods： Thirty healthy controls and forty newly diagnosed patients with lung cancer before treatment were enrolled for the detection of serum levels of 21 cytokines by liquid chip. The correlations between clinical characteristics of lung cancer and these cytokines were analyzed; in addition, the correlations between significantly differentially expressed cytokines were also analyzed.Results： The expressions of 11 cytokines, including GM CSF, IFN γ, IL 12（P70）, IL 1β, IL 2, IL 6, IL 7, TNF α, Fractalkine, IL 8, and MIP 3α（P〈0.05 or P〈0.01） in serum of patients with lung cancer were significantly higher than those in control group. However, there was no significant difference in the serum levels of 21 cytokines between non metastasis group and metastasis group of lung cancer patients. The serum expressions of IFN γ（P〈0.05） and MIP 1β（P〈0.05） in lung adenocarcinoma （AC） patients were significantly higher than that in squamous cell carcinoma （SCC） patients. And the expression of ITAC （P〈005） in SCC group was significantly higher than that in small cell lung carcinoma group （SCLC group）. The 11 highly expressed cytokines showed different correlations in two groups. Conclusion： The expressions of 11 cytokines were significantly increased in patients with lung cancer, including IL 6 and IL 8 etc. It suggested that they may be involved in the development of lung cancer. These highly expressed cytokines may be used for the diagnosis of lung cancer, and may provide new targeting for the research of lung cancer treatment.