香蕉根系丛枝菌根(AM)真菌染色方法比较

Comparison of Staining Methods on Arbuscular Mycorrhizal(AM) Fungi in Banana Roots

AM真菌侵染情况观察是AM真菌研究的重要基础。本研究比较了5种不同染色剂(5%醋酸墨水,酸性品红,苏丹红Ⅳ,台酚蓝,苯胺蓝)对香蕉根系AM真菌的染色效果。研究结果表明:5%醋酸墨水和台酚蓝染色液的染色效果最佳,根皮层细胞内的AM真菌的菌丝、丛枝、泡囊及内含物等结构清晰可见,且能够明确分辨出AM真菌与其他未知真菌。但综合考虑操作难易程度、价格成本和毒性等因素,5%醋酸墨水染色液更适用于香蕉根系AM真菌的染色和制片观察。

It is essential to establish a method for staining endophytic fungi in banana roots to observe arbuscular mycorrhizal （AM） fungi colonizations. An experiment was conducted to evaluate the banana roots dyed with five stains i.e. vinegar with 5% ink dye, acid fuchsin, sudan red IV, trypan blue, and aniline blue. The result showed that the structure of hyphae, arbuscular, vesicle and inclusion of AM fungi stained with both ink dye and trypan blue could be clearly visible. And AM fungi was distinguished from other unidentified fungi under microscope. The 5% ink-vinegar solution staining provided a simple, low toxic and inexpensive technique for staining AM fungi in banana roots with excellent staining results, and it was beneficial for observation and photograph, which had no oollution to the environment.