飞龙斩血内一株链霉菌属的分离鉴定及抑菌活性检测

Isolation,Identification and Bioactivity Screening of a Streptomyces Strain in Toddalia Asiatica L.

采用严格的表面消毒程序以及在培养基中添加抑菌剂的方法从中药植物飞龙斩血中分离得到一株内生放线菌F-3-003,菌株的发酵产物通过纸片扩散法体外检测具有一定的抑菌活性.菌落形态特征和23S rDNA序列分析表明,菌株F-3-003属于链霉菌属,通过16S rDNA序列分析显示菌株F-3-003与多株淡紫灰链霉菌(Streptomyces lavendulae)的序列同源性均高于99%,因此,将菌株F-3-003命名为淡紫灰链霉菌F-3-003,基因登录号为KF751667,本研究为飞龙斩血内生菌资源的开发利用提供了基础.

An endophytic actinomycetes strain F -3 -003 was obtained from T. asiatica, by the conventional method. It＇s detected that its fermentation broth had some antibacterial activity against gram -positive bac- teria by disc diffusion method. The strain F -3 -003 was classified and identified as streptomyces sp. based on its morphological characters and ribosomal 23S rDNA sequence analysis. The 16S rDNA sequence analy- sis homology between strain F - 3 - 003 and many other strains of streptomyces lavendulae was higher than 99%. So, the strain F- 3 -003 was named as streptornyces lavendulae F- 3 -003 while its gene accession number was numbered as KF751667. This research provides a basis for utilizing endophytic resourcesfrom T. asiatica.