PDK1对中性粒细胞分化的影响

Effect of PDK1 on Neutrophil Differentiation

目的:应用Vav-Cre在成体小鼠敲除PDK1并观察PDK1敲除对中性粒细胞比例、数目及分化的影响。方法:应用RT-PCR分析PDK1敲除后骨髓细胞PDK1表达水平;应用CFU-C实验检测PDK1对祖细胞功能的影响;应用流式细胞术分析PDK1对中性粒细胞比例的影响。结果:PDK1敲除后骨髓细胞PDK1表达水平很低;粒系祖细胞及巨噬祖细胞数目减少;中性粒细胞比例数目减少,但幼稚的中性粒细胞比例增多。结论:PDK1敲除影响了造血干细胞向祖细胞分化的过程,以及中性粒细胞的分化成熟过程。

Objective： To knock out PDK1 by using Vav-Cre and observe the effects of PDK1 knock out on the ratio, number and differentiation of neutrophil. Methods： The PDK1 expression level of Vav-Cre;PDKI mouse in the bone marrow cells was analyzed by RT-PCR. The effect of PDK1 on hematopoietic progenitor was observed by CFU- C assay, and the effect of PDK1 on the ratio and number of neutrophil was detected by flow cytometric analysis. Results ： The RNA expression level of bone marrow cells of Vav-Cre; PDK1 toxox mouse was dramatically lower than that of the control mouse. The number of functional GMP was lower in Vav-Cre;PDKIl mouse in contrast to controls. The percentage and number of neutrophil were lower, but the percentage of premyelocyte/myelocytes was more than 2 times in Vav-Cre; PDK1x/l group compared with control groups. Conclusion： PDK1 affects the process of the differention of hematopoietic stem cells to GMP, the neutrophil differention and maturation.