温阳活血方对慢性心力衰竭大鼠心肌纤维化的影响

Effect of Wenyang Huoxue prescription on myocardial fibrosis in rats with chronic heart failure

目的:探讨温阳活血方对慢性心力衰竭大鼠心肌纤维化的影响。方法:利用肾上腹主动脉缩窄法制作慢性心力衰竭大鼠模型。将30只雄性wistar大鼠随机分为假手术组、模型组、温阳活血方组,每组10只。温阳活血方组给予温阳活血方8m L/kg灌胃,其他两组给予同等剂量的0.9%氯化钠注射液灌胃,连续给药4周后观察各组大鼠左心室质量指数(LVMI)和胶原容积积分(CVF)水平,实时荧光定量(RT-PCR)测定各组大鼠左心室心肌结缔组织生长因子(CTGFmRNA)水平。结果:与假手术组比较,模型组LVMI、CVF及左心室心肌CTGFmRNA表达水平升高(P<0.05,P<0.01);与模型组比较,温阳活血方组LVMI、CVF及左心室心肌CTGFmRNA表达水平下降(P<0.05,P<0.01)。结论:温阳活血方能明显改善心肌纤维化,这可能与抑制CTGF过度表达有关。

Objective ：To investigate the effect of Wenyang Huoxue prescription on myocardial fibrosis in rats with chronic heart failure. Methods ： A rat model of chronic heart failure was established by coarctation of the supra- renal abdominal aorta. A total of 30 male Wistar rats were randomly divided into sham - operation group, model group,andWenyang Huoxue prescription group,with 10 rats in each group. The rats in the Wenyang Huoxue pre- scription group were given Wenyang Huoxue prescription 8 ml/kg by gavage, and those in the other two groups were given 0. 9% sodium chloride injection at the same dose by gavage. After administration for 4 consecutive weeks, left ventricular mass index（LVMI） and collagen volume fraction（CVF） were observed, and RT -PCR was used to meas- ure the mRNA expression of connective tissue growth factor（CTGF） in the left ventricle. Results：Compared with the sham -operation group,the model group had significant increases in LVMI, CVF, and mRNA expression of CTGF （P 〈0. 05,P 〈0. 01）. Compared with the model group, the Wenyang Huoxue prescription group had signifi- cantreductions in LVMI, CVF, and CTGF mRNA expression （ P 〈 0. 05, P 〈 0. 01 ）. Conclusion ： Wenyang Huoxue prescription can significantly improve myocardial fibrosis, possibly by inhibiting the overexpression of CTGF.