食管鳞状细胞癌中HER-2基因扩增和蛋白表达

HER-2 gene amplification and protein expression in esophageal squamous cell carcinoma

目的探讨食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)中HER-2基因扩增和蛋白表达状况以及与临床病理指标的关系。方法收集96例ESCC组织,采用免疫组化法检测HER-2蛋白表达,采用FISH法检测HER-2基因扩增,并对患者进行随访。结果免疫组化结果显示3例HER-2呈3+,15例2+,11例1+,67例-,3+的病例中2例HER-2基因扩增,15例2+的病例中1例拷贝数>6.0。HER-2蛋白过表达与基因扩增存在显著相关性(P<0.000 1)。HER-2基因扩增/蛋白过表达与肿瘤组织无坏死(P=0.012)、低临床分期(P=0.040)存在一定相关性。HER-2基因扩增/蛋白过表达患者呈预后好的趋势,但差异无统计学意义(P>0.05)。结论 HER-2在ESCC中有一定的基因扩增率和蛋白过表达率,两者具有相关性,且HER-2基因/蛋白过表达是潜在预后好的指标。

Purpose To explore the status of HER-2 gene amplification and protein expression in Chinese esophageal squamous cell carcinoma patients and its relationship with the clinicopathological parameters. Methods The HER-2 gene amplification was detected by FISH and protein expression by IHC in 96 patients with esophageal squamous cell carcinoma that would be followed up for 3 years. Results Three cases presented with 3 ＋ , 15 cases with 2 ＋ , 11 cases with 1 ＋, and 67 with nega- tive by HER-2 immunohistochemical staining; 2 of 3 cases presented with HER-2 amplification in in 3 ＋ , 1 case presented with over 6 HER-2 copy numbers. HER-2 amplification and HER-2 overexpression had significant correlation （ P 〈 0. 000 1 ）. HER-2 amplification or overexpression was associated with clinicopath- ological parameters, such as, carcinomas without necrosis （P = 0. 012） and lower stage （P =0. 040）. There was better DFS or OS trend in patients with HER-2 overexpression or amplification, but the significant difference did not reached. Conclusion This research has demonstrated the correspondence between gene amplification and protein overexpression of HER-2 in ES- CC. HER-2 gene overexpression or amplification is a potential better predictive parameter in ESCC, but needs further study.