鼠尾草叶绿体基因组序列分析

Complete Sequence Analysis of Chloroplast Genome of Salvia japonica

鼠尾草(Salvia japonica)是唇形科(Labiatae)鼠尾草属(Salvia)的一种多年生草本植物,具有十分重要的药用和经济价值。本文采用第二代测序技术Illumina Hiseq平台对鼠尾草的叶绿体基因组进行测序,同时以鼠尾草近缘物种丹参叶绿体基因组作为参考,组装得到完整叶绿体基因组序列。结果表明,鼠尾草叶绿体基因组序列全长153 995 bp,呈典型的四段式结构,其中LSC区长84 573 bp,SSC区长19 874 bp,两个IR区分别长24 774 bp;鼠尾草叶绿体基因组成功注释13组叶绿体基因,基因的种类、数目及GC含量等与唇形科中其它物种较为类似。这些研究结果丰富了鼠尾草属的叶绿体基因组数据,为今后鼠尾草属植物系统发育关系重建积累了基础性数据。

Salvia japonica is a perennial herb of the genus Salvia （ Lamiaceae）, which is of great medicinal and economic value. The chloroplast genome of S. japonica was sequenced using Illumina Hiseq platform. The complete chloroplast genome sequence was obtained by Salvia miltiorrhiza chloroplast genome as a reference. The full-length chloroplast genome of S. japonica was 153 995 bp. The LSC was 84 573 bp and 19 874 bp in SSC, and the length of IR region was 24 774 bp. The chloroplast genome of S. japonica successfully annotated 13 groups of genes. The gene types, number and GC content were similar with other species of Lamiaceae. These results enrich the chloroplast data and also provide some valuable molecular data for reconstructing evolutionary history of Salvia.