甘薯MADS-box转录因子IbMADS11-Like的克隆及胁迫响应分析

Cloning and Expression Analysis of Stress Treatments of a MADS-box Transcription Factor in Sweet Potato

MADS-box蛋白在植物生长发育及抗逆等过程中均发挥重要功能。本实验室根据甘薯近缘野生种I.trifida基因组序列,在甘薯栽培种徐薯22(Ipomoea batatas(L.)Xu22)中克隆到一个STMADS11亚家族MADS-box基因,命名为IbMADS11-Like。实时定量RT-PCR分析表明,IbMADS11-Like基因在甘薯根中大量表达,并且随着块根的形成和膨大表达量逐渐降低,表明该基因可能参与了甘薯块根的发育过程。胁迫处理分析表明,IbMADS11-Like基因的表达受干旱、盐和高温的诱导,而低温则抑制其表达。此外,IbMADS11-Like基因对ABA、IAA、ZT、BR、ACC、JA及GA等激素的处理也有不同程度的响应,暗示IbMADS11-Like基因可能参与了甘薯生长发育及胁迫的调控过程。这些结果为进一步分析IbMADS11-Like基因在甘薯块根发育和胁迫响应中的功能奠定了基础。

MADS-box proteins play important functions in the development and stress response of plants. A STMADSll subfamily gene was cloned from Ipomoea batatas （L.） Xu22 according to I. trifida genome sequences, named IbMADS11-Like. By real-time RT-PCR IbMADS11-Like was expressed abundantly in roots, and a rapid declining trend was observed as storage root formation and development. The IbMADS11-Like might be involved in the storage root development of sweet potato. Stress treatment experiments showed that IbMADS11-Like was induced by drought, NaC1 and high temperature, while was reduced by low temperature. ABA, IAA, ZT, BR, ACC, JA and GA treatments also impacted the expression of IbMADS11-Like, indicating that IbMADS11-Like might play functions in the development and stress response in sweet potato. These results play a foundation for further study on the physiological function of IbMADS11-Like in the development and stress response of sweet potato.