雪松松针挥发油化学成分及抗氧化活性研究

Chemical Components and Antioxidant Activity of Volatile Oil from Pine Needles of Cedrus deodara

采用水蒸气蒸馏法提取雪松松针挥发油,利用气相色谱-质谱联用技术分析其化学成分。使用维生素C为阳性对照,以DPPH自由基、ABTS自由基和羟基自由基清除作用为指标评价挥发油的抗氧化活性。从雪松松针挥发油中鉴定了37种化学成分,占挥发油总量的95.10%,以烃类和醇类为主;挥发油对DPPH自由基、ABTS自由基和羟基自由基有明显的清除作用,其IC50分别为0.30、0.22及0.96μg·mL^(-1),且呈现一定的量效关系。可见,雪松松针挥发油中含有多种有应用价值的化学成分,具有显著的抗氧化活性。

The volatile oil from pine needles of Cedrus deodara was extracted by water-steam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil was evaluated by DPPH · , ABTS · and · OH scavenging capacity with Vc as the positive control. Thirty-seven components, accounting for 95.10% of total components, were identified. Olefin hydrocarbon and alcohols were found to be dominant components in the volatile oil. The volatile oil exhibited significant scavenging capacity against DPPH · , ABTS· and · OH in a concentration fashion. When the scavenging rate against DPPH · , ABTS · and · OH of volatile oil was 50%, the contents of volatile were 0.30, 0.22 and 0.96 μg·mL^-1, respectively. The volatile oil from pine needles of C. deodara contains a variety of chemical composition of application value with a certain antioxidant activity.