高压均质法提取云芝胞内糖肽

Extraction of Intracellular Polysaccharopeptide from Coriolus versicolor Mycelium Using Hot Water and High-Pressure Homogenization

采用高压均质法提取云芝(Coriolus versicolor)胞内糖肽,与热水法进行比较。结果表明:高压均质提取的云芝胞内糖肽得率是热水提取的2倍,为(20.2±0.6)%;采用高压均质法提取的组分5相对百分含量最高,为48.07%;扫描电镜下观察发现,与热水提取相比,高压均质提取时云芝菌丝体破碎程度比较高。

High-pressure homogenization （HPH） and hot water extraction （HWE）were used to extract intracellular polysaccharopeptide （IP） from Coriolus versicolor mycelia. Yields of IP extracted using HPH reached （20.2± 0.6）%, which were twice as high as those achieved using HWE. HPLC separated the IPs into six components, of which the component with a peak retention time of 26.46 mins made up 48.07% of the material extracted using HPH. Scanning electron microscopy revealed that HPH-treated mycelium had a more porous and ruptured structure compared to HWE-treated samples.