四种鸡艾美耳球虫实验室保存株的PCR鉴定

PCR Identification of Laboratory Strains of Four Chicken Eimeria Species

以4种鸡球虫实验室保存株(毒害、巨型、柔嫩、堆型艾美耳球虫)的基因组DNA为模板,分别用针对4种球虫的ITS-1序列设计的种特异性引物进行PCR扩增,扩增产物进行琼脂糖凝胶电泳分析,观察特异性条带。结果显示,只有在模板和扩增引物为同一种球虫时,方能扩增出特异性条带。表明本实验室保存的4种鸡球虫均为纯种株,建立的PCR检测方法可以用于鸡球虫纯种的鉴定。

The genome DNAs extracted from oocysts of Eimeria necatrix, E. maxima,E, ten.ella and E. acervulina, were used as templates for PCR with four sets of species-specific primers designed according to the previously published ITS-1 sequences of these four species of coccidian, respectively. The PCR products were analyzed using agarose gel electrophoresis. The results showed that the species-specific DNA would be amplified when both the templates and primers used in PCR come from the same Eimeria species. It concluded that these four avian coccidia were the pure strains and PCR can be used to identify species and purity of strains.