从猪胰脏中提取及纯化羧肽酶B工艺改进

STUDY ON EXTRACT AND PRUIFICATION OF CARBOSYPEPTIDASE B FROM PORCINE PANCREAS

从猪胰脏中提取及纯化羧肽酶B。采用匀浆、盐析的方法进行初步提取及纯化 ,采用疏水层析、离子交换层析的方法进行进一步纯化。纯化后羧肽酶B比活大于 2 0 0U/mg ,收率为 5 0U /g胰脏。结论该方法周期短 ,收率高 。

Carboxypeptidase B is purifed from porcine pancreas. Primary purification is achieved through homogenization and salting out, and further purifying by using HIC and IEC.After purification, the specific activity of carboxypeptidase B is 200 Units/mg protein.The productive rate is 380Units/g porcine pancreas.The new method has advantages of short processing period,high productive rate,good repeatability.