鸦胆子水溶性成分的含量测定及提取工艺

Content Determination and Extraction Process of Water-soluble Component in Brucea Javanica

目的:建立鸦胆子水溶性成分的含量测定方法,优选其提取工艺。方法:采用HPLC法,以尿嘧啶为对照品,测定其含量,并利用正交设计优选最佳提取工艺。结果:尿嘧啶浓度在0.0240~0.0960 mg/m L范围内线性关系良好,回归方程为Y=1.3074X+679.24,r=0.9954,RSD=1.11%(N=6)。优化的提取工艺条件为药材6倍量蒸馏水,100℃提取60 min。结论:该测定方法简单,提取工艺稳定可行,可为鸦胆子的研究提供参考。

Objective To set up a method for the determination of water-soluble component in Brucea Javanica, and optimize the extraction process. Methods With the Uracil as reference, the water-soluble component in Brucea Javanica was determined by HPLC. The experiment was carried out by orthognnal design to optimize the extraction process. Results Uracil showed good linearity in the range of 0.0240-0.0960 mg/mL, r=0：9954, regression equation was Y=1307.4X＋679.24, and relative standard deviation （RSD） Was 1.11% （N=6）. The optimized process condition was the liquid-solid ratio being 6： 1, and extraction for 60 min at 100 ℃.Conclusion The established method is simple, The extraction process is stable and feasible, and can supply evidence for its further study.