正交法优化连翘叶中连翘酯苷的超声提取工艺

Orthogonal Optimization of Ultrasonic Extraction Technology of Forsythiaside in Forsythia Suspense Leaves

目的:优选连翘叶中连翘酯苷的超声提取工艺。方法:以连翘酯苷的得量为指标,以连翘酯苷为对照品,采用超高效液相色谱法测定超声法提取连翘叶中连翘酯苷的含量。通过L_9(3~4)正交试验,对影响超声法提取连翘酯苷效果的四个因素(提取温度、提取时间、提取频率、提取次数)进行优选。结果:连翘叶在提取温度50℃、提取时间32 min、超声频率35 k Hz,提取1次即可达到最佳提取效果。结论:本实验为优化连翘叶中连翘酯苷的超声提取工艺提供了参考依据。

Objective： To optimize the ultrasonic extraction technology of Forsythia suspense leaves. Method： The weight of the obtained Forsythiaside was as the standard. Use Forsythiaside as control samples. With UPLC of ultrasonic extraction of Forsythiaside in Forsythia suspense leaves. Using L9（3^4） orthogonal test, the tour factors （ extraction temperature, extraction time, extraction frequency and extraction times ） wonld be selected, which influenced the ultrasonic extraction of Forsythiaside from Forsythia suspense leaves. Result： The extraction effect of Forsythia suspense leaves was the best in the extraction temperature 50 ℃, extraction time 32 rain, ultrasonic frequency 35 kHz. Conclusion： The experiment for the optimization of ultrasonic extraction of Forsythiaside frona Forsythia suspense leaves provides reference.