不同形态无机氮对蛋白核小球藻生长及碳酸酐酶胞外酶活性的影响

Effect of different forms of inorganic nitrogen on algae growth and extracellular carbonic anhydrase in Chlorella pyrenoidosa

为研究不同形态无机氮对湖泊富营养化的影响,本研究采用实验室内纯培养微藻来模拟不同浓度铵态氮、硝态氮对蛋白核小球藻生长及其对碳酸酐酶胞外酶活性的影响。研究发现:提高无机氮浓度有利于蛋白核小球藻的生长,且铵态氮更利于蛋白核小球藻的生长。碳酸酐酶胞外酶在补偿蛋白核小球藻的无机氮营养不足方面具有重要作用。

In order to study the effect of different forms of inorganic nitrogen on lake eutrophication,in this study,Chlorella pyrenoidosa was cultured in a greenhouse by adding different concentrations of ammonium nitrogen or nitrate nitrogen in the medium. The growth rate and the extracellular carbonic anhydrase activity were collected under different concentrations or forms of inorganic nitrogen.From this study,we conclude that the growth of the inorganic nitrogen is conducive to Chlorella pyrenoidosa,and ammonium nitrogen is more active than that of nitrate nitrogen. The extracellular carbonic anhydrase plays an important role in compensating inorganic nitrogen deficiency for Chlorella pyrenoidosa.