小鼠脑缺血再灌注模型的优化及其效果评价

Optimization and evaluation of cerebral ischemia reperfusion model in mice

目的:通过结扎颈内动脉的方法对小鼠脑缺血再灌注模型进行优化并进行效果评价。方法:选取健康雄性昆明小鼠90只,随机分为假手术组(Sham组)、结扎颈内动脉组(LICA组)、结扎颈外动脉组(LECA组)3组,每组30只。术后24 h对各组小鼠行神经功能缺损评分(NSS)评价模型的成功率,2,3,5-氯化三苯基四氮唑(TTC)染色测定脑梗死体积比,并对小鼠脑梗死体积比与行为学评分的相关性进行评价;同时分别在术前及术后24 h、72 h、1周测量小鼠体重及Clark一般功能损伤评分,用于评价小鼠一般生存状态。结果:术后24 h,TTC染色证实LICA术式可诱发明显的局灶性脑缺血且模型成功率达到93.3%。与LECA术式相比,应用LICA术式,NSS与TTC染色之间具有更好的一致性,TTC染色测定小鼠脑梗死体积比与NSS评分具有显著的相关性;与LECA术式相比,应用LICA术式,术后24 h、72 h、1周小鼠的体重变化率、死亡率、Clark一般功能损伤评分均降低。结论:小鼠脑缺血再灌注模型中,应用LICA术式可提高模型的成功率,提高梗死病灶大小与行为学评分的相关性,提高小鼠生存率及一般功能状态。

Objective： To optimize and evaluate the cerebral ischemia reperfusion model of mice by means of ligation of internal carotid.Methods： Ninety healthy male Kunming mice were randomly divided into 3 groups with 30 in each group,i.e., sham operation group（Sham）, ligation of internal carotid artery（LICA） group and ligation of external carotid artery（LECA） group.Assessment for neurological severity scores（NSS） was conducted to evaluate the success rate of the model and 2,3,5-triphenyhe trazolium chloride（TTC） staining was used to measure the volume ratio of cerebral infarct 24 h after operation.The correlation between the volume ratio of cerebral infarct and NSS was evaluated.The mice weight was measured and Clark＇s general deficits scoring was performed to evaluate the mice general survival condition 24 h,72 h,1 w after operation, respectively.Results： TTC staining confirmed that LICA method could induce evident focal cerebral ischemia, and the success rate of model reached 93.3% 24 h after operation.Compared with the LECA method, there was a better coherence between NSS and TTC staining in LICA group, as well as a significant correlation between the volume ratio of cerebral infarct measured by TTC staining and NSS.The mortality of mice 24 h, 72 h and 1 w after operation was lowered by the use of LICA surgical method and the rate of change in body weight of mice was reduced, so was the Clark＇s general deficit score.Conclusion： In the cerebral ischemia reperfusion model of mice, the application of LICA surgical method can improve the success rate of model and the survival rate and general functional status of mice, and increase the correlation between size of infarct focus and NSS.