人毛乳头细胞的冻存

Study on Freezing Storage of Human Dermal Papilla Cells

寻找一种不影响人毛乳头细胞生物活性的冻存方法。采用标本沉降式液氮冻存方法 ,冻存新分离的人毛乳头 ;在冻存 1月、3月和 6月时解冻复苏 ,接种培养 ,观察毛乳头的贴壁率和细胞生长情况。复苏冻存了 1月、3月和 6月的毛乳头 ,培养 2天时毛乳头贴壁率为 90 %～ 95 %左右 ,与新分离的毛乳头贴壁率相近 ;瓶内细胞融合时间也基本相同 ,为 15天。液氮冻存人毛乳头不会影响其细胞生物活性 。

To explore a safety method of freezing storage of human dermal papilla cells not influencing its biological activity. The enormous dermal papillae isolated newly were freezed with cryoprotective agent including dimathyl sulfoxide and storaged in liquid nitrogen after sinking gradually. At the 1 month, 2 month and 6 month after submersing in liquid nitrogen, the freezing dermal papillaes got revivfication after thawing, inoculated and raised in Dulbecco's Modified eagle's medium. The rate of dermal papillaes attaching to the wall and the cell growth behaviour were observed. Similar to that of islated newly, the rates of dermal papillaes attaching to the wall at 2 days after culture were 90 percents to 95 percents after freezing for 1 month, 2 months and 6 months. The dermal papillae also can culture for dermal papilla cell which exhibites a polygonal cellular morphology and a tendency to form pre-confluent multi-layered cluster after restored in liquid nitrogen. It was approximately 15 days to approach cell fusion in culture bottle. Conclusion It is suggested that freezing storage of human dermal papillaes has no side-effect in their biological characteristics and is worth application in the series experiment of DPC .