ALOX5AP基因单核苷酸多态性与缺血性脑卒中患者颈动脉斑块的相关性分析

Correlation of ALOX5AP gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke

目的探讨5-脂氧合酶激活蛋白(ALOX5AP)基因单核苷酸多态性与缺血性脑卒中患者颈动脉斑块的关系,为颈动脉斑块的缺血性脑卒中早期诊断、治疗和发病机制研究提供依据。方法选取737例缺血性脑卒中患者,按有无颈动脉斑块分为斑块组和无斑块组,应用多重聚合酶链反应(PCR)及基因序列分析技术分析各组患者ALOX5AP基因8个位点多态性分布情况。结果斑块组的甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、非高密度脂蛋白胆固醇(non-HDL-C)、收缩压和血葡萄糖(Glu)水平均明显高于无斑块组(P<0.05)。对ALOX5AP基因8个位点基因型和等位基因频率分析发现,斑块组与无斑块组间差异无统计学意义(P>0.05)。ALOX5AP基因8个位点组成的2个单倍型构成在斑块组与无斑块组间差异无统计学意义(P>0.05)。结论 ALOX5AP基因多态性与缺血性脑卒中患者颈动脉斑块形成无关。

Objective To study the correlation of 5-lipoxygenase activating protein（ALOX5AP）gene single nucleotide polymorphisms and carotid plaques in patients with ischemic cerebral stroke,and to provide a reference for the early diagnosis,treatment and pathogenesis of carotid plaque-related ischemic cerebral stroke.Methods A total of 737 patients with ischemic cerebral stroke were enrolled and classified into carotid plaque group and non-carotid plaque group. Multiple polymerase chain reaction（PCR） and gene sequencing were used to analyze the 8 locus polymorphisms of ALOX5AP gene.ResultsTriglyceride（TG）,low-density lipoprotein cholesterol （LDL-C）,non-high-density lipoprotein cholesterol（non-HDL-C）,systolic blood pressure and blood glucose （Glu） levels in carotid plaque group were higher than those in non-carotid plaque group（P〈0.05）. There was no statistical significance for the genotypes and allele frequencies of 8 loci of ALOX5AP gene between carotid plaque and non-carotid plaque groups（P〉0.05）. There was no statistical significance for 8 loci of ALOX5AP gene in the formation of 2 haplotypes between carotid plaque and non-carotid plaque groups（P〉0.05）.Conclusions ALOX5AP gene polymorphisms have no relationship with carotid plaque formation in ischemic cerebral stroke.