基于光解笼锁谷氨酸的离体癫痫模型建立

Establishment of the epilepsy model in vitro based on optical release of caged-glutamate

目的:建立离体癫痫模型模拟癫痫发作,更好地研究癫痫发病的内在机制。方法:针对目前电刺激和药物干预存在的缺陷,建立了基于光解笼锁谷氨酸的神经元细胞刺激系统,并以培养在微电极阵列(MEA)上的海马神经元作为研究对象。特定波长的紫外光经光学聚焦系统聚焦后,照射到笼锁谷氨酸电解液中,解除光敏基团的笼锁作用,释放出谷氨酸。结果:紫外光未作用时海马神经元呈现正常的电位发放;紫外光激发后释放出谷氨酸,海马神经元呈现癫痫样放电。结论:初步建立了离体癫痫模型,为癫痫发病机制的研究提供了理性的离体模型。

Objective： In order to better study the internal mechanism of the seizure, the epilepsy model in vitro was established to simulate seizure discharge. Methods： Aiming at the drawbacks of the electrical stimula-tion and drugs intervention, a stimulation system of neurons based on optical release of caged-glutamate was developed. The hippocampal neurons cultured on the microelectrode array were used as the research object. The ultraviolet （UV） focused by the optical focus system was delivered to the bath solution contained cage-glutamate. Using a specific wavelength of UV to remove the caged function of photosensitive groups, glutamate was re-leased. Results： The hippocampal neurons showed the normal potential when the UV did not work. The gluta-mate induced seizure discharge in the hippocampal neurons after the UV excitation. Conclusion： The epilepsy model in vitro was preliminarily set up. The model provides the rational model in vitro for the researches on the mechanism of the epilepsy.