miR-93、miR-195、miR-196a表达与胃癌患者化疗疗效关系研究

Correlation of serum miR-93,miR-195,miR-196a levels and chemotherapy efficacy of patients with gastric cancer

目的探讨血清微小RNA 93(miR-93)、微小RNA 195(miR-195)、微小RNA 196a(miR-196a)表达水平与新疆汉族与维吾尔族胃癌患者化疗疗效的相关性。方法收集2009年1月-2011年6月于新疆医科大学附属肿瘤医院接受治疗的新疆地区进展期胃癌患者213例,其中汉族97例,维吾尔族116例,另选同期于我院进行健康检查的正常人178例作为对照组。所有患者均接受紫衫类+奥沙利铂+替吉奥方案治疗,并于化疗前及2个疗程化疗后采集静脉血提取RNA,应用实时荧光定量聚合酶链式反应定量分析miR-93、miR-195和miR-196a的表达水平。按照实体瘤评价标准1.1(RECIST 1.1)将患者根据疗效分为有效组(部分缓解+完全缓解)和无效组(稳定+进展),每3个月进行随访,分析miR-93、miR-195、miR-196a的表达水平与化疗疗效及预后的关系。结果化疗后miR-93、miR-195表达水平均较化疗前明显升高,而miR-196a表达水平明显降低(P<0.05)。有效组患者miR-93及miR-195的表达水平均明显高于无效组,而miR-196a表达水平明显低于无效组(P<0.05)。汉族和维吾尔族患者miR-93、miR-195和miR-196a的表达无差异(P>0.05)。miR-93和miR-195高表达患者的生存时间均明显高于低表达患者,而miR-196a低表达患者的生存时间明显高于高表达患者(P<0.05)。COX回归模型表明淋巴结转移、miR-93、miR-195、miR-196a表达水平是胃癌患者的独立预后因素。结论miR-93、miR-195和miR-196a与胃癌患者的化疗疗效和预后有关,汉族、维吾尔族患者未见差异,是潜在的胃癌预后生物标志物。

Objective To investigate the correlation of serum microRNA93,microRNA195,microRNA196a levels with chemotherapy efficacy of patients with gastric cancer in Uyghur and Han ethnics. Methods A total of 213 patients （116 cases in Uyghur, 97 cases in Han） with gastric cancer in Xinjiang who received treatment at the Affiliated Tumor Hospital of Xinjiang Medical University between January 2009 and July 2011 were selected. 178 healthy people received physical examination in the hospital at the same time werealso collected as control. All patients were received Paclitaxel ＋ oxaliplatin ＋ S-1 chemotherapy treatment regimen, and venous blood was collected before chemotherapy and 2 courses after chemotherapy for ex-traction of RNA. qRT-PCR was applied for testing expressions of microRNA93, microRNA195 and mi- croRNA196a. All patients were divided into effective group （Partial remission＋complete remission） and ineffective group （Stability＋progress） according to RECIST 1.1 criteria. Follow-up was conducted every 3 months for analyzing the correlation of serum microRNA93/microRNA195/microRNA levels and chemo-therapy efficacy and prognosis of patients with gastric cancer. Results After chemotherapy, microRNA93 and microRNA195 expressions were higher than those before chemotherapy, while microRNA196a expres-sion was lower （P 〈0.05）. Compared with the ineffective group, patients in the effective group had higher microRNA93 and microRNA195 expressions and lower microRNA196a expression （P 〈0.05）. No signifi-cant difference was found in comparisons of microRNA93, microRNA195 and microRNA196a expressions between the Uyghur and Han groups （P〉0.05 ） . The survival time of patients with high expressions of microRNA93 and microRNA195 was longer than those with low expressions of microRNA93 and microR- NA195, while patients with low expression of microRNA196a had longer survival time than those with high expression of microRNA196a （P〈0.05）. COX regression analysis showed that lymph node metasta-sis, microRNA93, microRNA195 and microRNA196a expressions were the independent prognostic factors for patients with gastric cancer. Conclusion microRNA93, microRNA195 and microRNA196a are associ-ated with the curative effect and prognosis of gastric cancer patients, and there is no difference between the Uyghur and Han groups, which are potential biomarkers of gastric cancer prognosis.