紫外联合红外光谱指纹图谱整合评价三黄片质量

The Integrated Use of Ultraviolet Spectrum Fingerprint Coupled with Infrared Spectrum Fingerprint to Comprehensively Assess the Quality Grades of San-huang Tablets

目的:整合评价不同来源的30批三黄片样品质量。方法:建立三黄片紫外指纹图谱和红外指纹图谱检测方法,将两种分析方法结果取均值整合后评价样品质量。结果:整合后的大部分样品质量等级均在合格范围内(质量等级≤5)。结论:任何一种检测方式都具有单一片面性,不能全面表征样品真实质量,需采用多种分析方法并用、全方位整合的技术手段才能客观综合地对其进行质量评价,为中药在其复杂组分未完全分离的情况下,提供了一种简捷快速、全面客观的定性定量质控研究方法。

Objective: To comprehensively evaluate the quality grades of 30 batches of San-huang tablets from diverse commercialbrands. Methods: Ultraviolet spectrum fingerprint and infrared spectrum fingerprint were developed for the measurement of San-huang tablets. To evaluate the quality of the drug samples, SL, MLand α values were computed for each analytical method, and theintegrated SL, MLand α values were also calculated by taking the average value of the above-established method. Results: Most of thesamples were qualified(quality grade≤5)after the use of integrated method. Conclusion: Any detection method is single and one-sided, and could not overall characterize the tested samples, which reminds us that it is essential to adopt multi-approaches to disclosethe quality information of traditional Chinese medicine and herbal preparations. The strategy proposed in this study provides a simple,fast, comprehensive and objective approach for the quality control of traditional Chinese medicine and herbal preparations from boththe qualitative and quantitative aspects, in the case that complex components are not yet completely separated.