蓖麻RcNHX3基因的克隆与生物信息学分析

Analysis of Cloning of RcNHX3 Gene in Castor(Ricinuscommunis L.)

液泡膜型Na^+/H^+逆向转运蛋白基因(NHX)是植物抵御盐害的关键基因.本研究利用RACE方法克隆了蓖麻Rc NHX3基因的3'端和5'端片段.最后设计全长引物获得蓖麻RcNHX3全长序列,并对其进行生物信息学分析.结果表明,蓖麻液泡膜型钠氢逆向转运蛋白基因的c DNA完整的开放阅读框序列为1611bp,推测其可编码536个氨基酸;含有Na+/H+交换泵(^(50)NES^(52))是液泡膜型Na^+/H^+逆向转运蛋白重要的特征之一;存在12个跨膜结构域,属于跨膜蛋白;预测NHX3蛋白的二级结构和三级结构.

The vacuole membrane sodium hydrogen reverse transporter （Tonoplast Na＋/H＋ antiperters, NHX） isa key gene for plant resistance to salt damage.RACE methods were used to clone the segments of the 3 ＇ and 5＇ ends of the castor RcNHX3 gene.The full-length castor RcNHX3 sequence was obtained using the full length primers. The results show that the complete open reading frame sequence of eDNAin the vacuolar membrane sodium hydrogen reverse transfer gene of Castor was 1611 bp, speculating that the encoding 536 amino acids and the Na＋/H＋ exchange pump （50NES52） are one the most important characteristics of vacuole membrane the Na＋/H＋ reverse transporters. There are 12 transmembrane domains truetures which belong to transmemhrane proteins and the secondary structure and tertiary structure of NHX3 protein are predicted.