小剂量叶酸预防妊娠期高血压有效剂量的实验研究

An Experimental Study on the Optimal Dose of Folic Acid Intake to Prevent the Gestational Hypertension

目的探讨不同剂量叶酸对高同型半胱氨酸妊娠期SD大鼠血清叶酸及血浆同型半胱氨酸(Hcy)水平的影响作用,找出预防妊娠期高血压的最佳有效剂量。方法将40只12周龄雌性SD大鼠按照叶酸干预剂量分为4组:低、中、高3个剂量叶酸组[0.8mg/(kg·d),n=10],中剂量叶酸组[1.6mg/(kg·d),n=10]、高剂量叶酸组[2.0mg/(kg·d),n=10]和对照组(灭菌水500μL,n=10)。干预7d后,将雌鼠和同品类雄鼠合笼,第2~3天进行阴道栓检测,将受孕大鼠纳入研究,采用1%蛋氨酸饮食建立高同型半胱氨酸模型,干预前后分别抽取血液样本,测定各组血清叶酸及血浆Hcy水平。结果 32只雌鼠受孕并纳入干预研究,4组SD大鼠体质量在干预前后比较,差异均无统计学意义(P>0.05);中剂量叶酸组、高剂量叶酸组与对照组比较,叶酸水平升高,差异具有统计学意义(P<0.05);与对照组比较,高剂量叶酸组SD大鼠Hcy水平降低,差异具有统计学意义(P<0.05)。结论不同剂量叶酸干预效果存在差异,在安全剂量下,高剂量组叶酸能够提高妊娠期SD大鼠的叶酸水平和降低Hcy水平,从而达到预防妊娠期高血压的目的。

Objective To explore the effects of different doses of folic acid intake among pregnant SD mice with hyper-homocysteine and investigate the optimal dose for gestational hypertension.Methods 40 female SD mice with the age of 12 weeks were selected in the study and divided into four groups including high dose group （2.0 mg/kg＆#183;d）,medium dose group （1.6 mg/kg＆#183;d）,low dose group （0.8 mg/kg＆#183;d）and the control group,and each group consisted of 10 mice.The cohabitation of those female mice and the other male mice of same category was made after 7-day intervention.Then the vaginal suppository test was adopted after 2 to 3 days,and the pregnant mice were selected to create the hyper-homocysteine model with 1% methionine.The blood samples were collected before and after intervention respectively and assayed to determine the levels of serum folate and homocysteine. Results 32 pregnant mice were included in the intervention study.The weights of the SD mice in the three groups of low,medium and high doses were not significantly different from those in the control group before intervention. After the intervention,the levels of serum folate in the groups of medium and high doses were significantly higher than those in the control group （P〈0.05）;the level of homocysteine in the high dose group was significantly lower than that in the control group （P〈0.05）.Conclusion The intervention effects are different among the different doses of folic acid intake,and the high-dose intake of folic acid could increase the level of serum folate in pregnant SD mice and decrease the level of serum homocysteine for the purpose of preventing gestational hypertension with safe dose.