摘要
Biomedical applications of porous calcium car- bonate （CaCO3） microspheres have been mainly restricted by their aqueous instability and low remineralization rate. To overcome these obstacles, a novel symmetry-breaking assembled porous calcite microsphere （PCMS） was con- structed in an ethanol/water mixed system using a two-step vapor-diffusion/aging crystallization strategy. In contrast to the conventional additive-induced crystallization method, the present strategy was performed under mild conditions and was free from any foreign additives, thus avoiding the potential contamination of the final product. Meanwhile, the prepared PCMSs were characterized by their highly uniform spherical morphology and large open pores, which are fa- vorable for large protein delivery. An antimicrobial study of immunoglobulin Y （IgY）-loaded PCMSs revealed excellent antimicrobial activity against Streptococcus mutans. More importantly, they showed surprisingly rapid transformation to bone minerals in physiological medium. Evaluation of the in vitro efficacy of PCMSs in dentinal tubule occlusion demonstrated their powerful potential to serve as a catalyst in the repair of dental hard tissue. Therefore, the developed PCMSs show great promise as multifunctional biomaterials for dental treatment applications.
多孔碳酸钙微球的弱水溶液稳定性和低再矿化速率限制了其在生物医药领域的应用.为了解决该问题,本论文通过"气体扩散-陈化"两步策略制备了具有大孔结构和方解石相的碳酸钙微球材料.该合成策略反应条件温和,同时不需要使用模板剂和晶型导向剂,能够确保微球产物的生物安全性.所制备的碳酸钙微球可以吸附大量的免疫球蛋白IgY,并表现出增强抑制口腔变形链球菌的能力.此外,研究显示该微球可以在水溶液中保持较高的结构稳定性,而在人体体液和唾液环境中快速再矿化并转变成羟基磷灰石结构,对牙本质小管具有较佳的封闭效果,为其用于治疗牙本质过敏症提供了依据.
基金
supported by the National Natural Science Foundation ofChina (51402329 and 81500806)
the Science Foundation for Youth Scholar of State Key Laboratory of High Performance Ceramics and Superfine Microstructures (SKL201404)
Shanghai Excellent Academic Leaders Program (14XD1403800)
