Hydrophobe-substituted bPEI derivatives: boosting transfection on primary vascular cells

疏水基取代的分枝状聚乙酰亚胺衍生物:促进原发性血管细胞中的转染（英文）

Gene therapy targeted to vascular cells repre- sents a promising approach for prevention and treatment of pathological conditions such as intimal hyperplasia, in-stent and post-angioplasty restenosis. In this context, polymeric non-viral gene delivery systems are a safe alternative to viral vectors but a further improvement in efficiency and cytocom- patibility is needed to improve their clinical success. Herein, a library of 24 branched polyethylenimine （bPEI） derivatives modified with hydrophobic moieties was synthesised, char- acterised and tested in vitro on primary vascular cells, aim- ing to identify delivery agents with superior transfection effi- ciency and low cytotoxicity. Low molecular weight PEIs （0.6, 1.2 and 2 kDa） were grafted with long （C18） and short （C3） aliphatic chains, featuring different unsaturation degrees and degrees of substitution. 0.6 kDa bPEI-based derivatives were generally ineffective in transfection on vascular smooth mus- cle cells （VSMCs）, while among the other derivatives some promising vectors were identified. Forcing polyplexes on the cell surface by means of centrifugation invariably boosted transfection levels but increased cytotoxicity as well. Of note, a propionyl-snbstituted derivative （PEI2-PrA1, C3：0） was the most effective on both VSMCs and endothelial cells （ECs）, with higher and more sustained gene expression in combi- nation with markedly lower cytotoxicity with respect to the gold standard 25 kDa bPEI. In addition, a linoleoyl-substi- tuted derivative （PEI1.2-LA6, C18：2） owing to its high effi- ciency in VSMCs and relative inefficacy in ECs, combined with tolerable cytotoxicity was proposed as a vector for spe- cific VSMCs targeting.

针对血管细胞的基因治疗代表了一种有望用于预防和治疗内膜增生、血管支架狭窄和血管成形术后狭窄等病理状态的方法.聚合物非病毒载体的基因传递系统可以安全替代病毒载体,但是为了提高临床效果,它们的治疗效率及细胞相容性还需要进一步改善.本文合成了一系列24种被疏水基团修饰的分枝状聚乙酰亚胺衍生物(bPEI),并进行了表征及在体外原发性血管细胞内的测试,旨在筛选出具有优异的转染效率和低细胞毒性的传递剂.低分子量的聚乙酰亚胺(0.6,1.2 and 2 kDa)以不同取代程度接枝上了不同饱和度的长(C18)和短(C3)的不饱和脂肪链.丙酰取代衍生物(PEI2-PrA1,C3:0)在血管平滑肌细胞和内皮细胞转染中是最有效的,与著名的黄金标准25 kDa bPEI相比,具有更优异、更持久的基因表达,且毒性更低.此外,亚油酰基取代衍生物(PEI1.2-LA6,C18:2)由于在血管平滑肌细胞转染过程中效率高,而在内皮细胞中相对无效,且其具有可容忍的细胞毒性,可作为特定靶向于血管平滑肌细胞的载体.