长链非编码RNA在类风湿性关节炎患者和健康人PBMCs中差异表达及其发病机制

Differential expression of long chain noncoding RNA in PBMCs in health people and patients with rheumatoid arthritis and its pathogenesis

目的探究长链非编码RNA(IncRNA)在类风湿性关节炎(RA)患者外周血单个核细胞(PBMCs)和健康人PBMCs中的差异表达,并对其分析以进一步阐明RA的发病机制。方法选取2016年1-4月在该院风湿免疫科就诊的RA患者22例作为观察组,22例健康人作为对照组。采用Agilent Human IncRNA芯片测定5例RA患者和5例健康人的PBMCs中的IncRNA和mRNA的差异表达;利用GO以及Pathway分析检测出差异表达的IncRNA的功能分布,构建起IncRNA-mRNA的共表达网络,采用Trans-与Cis-预测其中与RA发病相关的IncRNA。结果 IncRNA在RA患者的PBMCs和健康人的PBMCs中共存在1 623条差异表达,mRNA差异表达则有851条。GO、Pathway分析表明,差异表达的mRNA的主要功能是参与蛋白激酶、核苷酸、金属离子的结合和转录调节,还参与B细胞受体信号通路、TNF信号通路的调节。经过Cis-和Trans-分析预测和Real-time PCR验证后找到3个IncRNA可能与RA发病有关,分别为NONHSAT 120696、uc.80+、NONHSAT 031501。结论 IncRNA在RA患者PBMCs和健康人PBMCs中存在差异表达,NONHSAT 120696、uc.80+、NONHSAT 031501 3个IncRNA可能与RA的发病有关。

Objective To explore the differentia expression of long chain noncoding RNA （IncRNA） in peripheral blood mononuelear cells （PBMCs） of the patients with rheumatoid arthritis （RA） and normal human and to analyze to further clarify the pathogenesis of RA. Methods Twenty-two cases of RA in our hospital from January to April 2016 were selected as the observation group and 22 healthy persons served as the control group. The differential expression of IncRNA and mRNA in PBMCs of 5 RA ca- ses and 5 healthy persons were determined by using Agilent human IncRNA chip;GO and Pathway were used to analyze the func- tional distribution of differentially expressed IncRNA,the co-expression pressionsnetwork was constructed and the Trans - and Cis - were adopted to predict the RA onset related IncRNA. Results There were 1623 differential expressions of IncRNA in PBMCs of RA patients and healthy persons and 851 differential expressions of mRNA. GO and Pathway analysis showed that the main func- tion of differentially expressed mRNA was to participate in the binding and transcriptional regulation of protein kinase, nucleotide and metal ions, and also participate in the regulation of B cell receptor signaling pathway and TNF signaling pathway. After Cis- and Trans- analysis prediction and real-time PCR validation, the 3 IncRNA could be related with RA onset, which were NONHSAT 120696,uc. 80＋ and NONHSAT 031501. Conclusion IncRNA has differential expression in PBMCs of RA patients and healthy persons, three 031501 IneRNA of NONHSAT 120696, uc. 80＋, NONHSAT may be related to the pathogenesis of RA.