肺腺癌患者外周血组蛋白去乙酰化酶1与血管内皮生长因子表达的变化及临床意义

Changes and clinical significance of peripheral blood HDAC1 and VEGF expression in patients with lung adenocarcinoma

目的探讨肺腺癌患者组蛋白去乙酰化酶1(HDAC1)与血管内皮生长因子(VEGF)表达的相关性。方法以该院2014年8月至2016年4月收治的80例肺腺癌患者为研究对象(观察组),另以同期该院健康体检者80例为对照组,收集所有研究对象空腹静脉血标本,采用酶联免疫吸附试验(ELISA)检测血清HDAC1、VEGF表达水平,并比较两组血清HDAC1、VEGF表达水平差异;分析不同特征的肺腺癌患者HDAC1、VEGF表达水平和肺腺癌患者血清中HDAC1和VEGF浓度的关系,并分析肺腺癌患者HDAC1蛋白表达水平的可能影响因素。结果对照组和观察组HDAC1水平分别为(329.56±23.83)、(568.20±35.40)ng/L,比较差异有统计学意义(t=23.576,P=0.000)。对照组和观察组VEGF水平分别为(40.26±9.82)、(296.56±19.80)ng/L,差异有统计学意义(t=31.154,P=0.000)。不同性别、年龄、临床分期和吸烟史的患者HDAC1蛋白水平差异均有统计学意义,男性,年龄大于60岁,临床分期Ⅲ、Ⅳ期和有吸烟史的患者HDAC1蛋白水平较高(P<0.05)。Pearson相关分析结果显示,肺腺癌患者血清中HDAC1和VEGF蛋白浓度呈正相关(r=0.526,P=0.000)。Logistic回归分析显示肺腺癌患者HDAC1蛋白表达水平的影响因素为临床分期。结论肺腺癌患者HDAC1蛋白的高表达可能同时调节VEGF的表达,从而促进肺腺癌的发生、发展。

Objective To investigate the correlation between histone deacetylase I（HDAC1） and vascular endothelial growth factor（VEGF） in the patients with lung adenocarcinoma. Methods Eighty cases of lung adenocarcinoma in our hospital from August 2014 to April 2016 served as the research subjects,and contemporaneous 80 individuals undergoing healthy physical examina- tion were taken as the control group. The fasting venous blood sample was collected in all subjects. Then serum HDAC1 and VEGF levels were detected by ELISA. The differences of serum HDAC1 and VEGF expression levels were compared between the two groups. The HDAC1 and VEGF expression levels in the patients with different characteristics of lung adenocarcinoma and the relation between serum HDAC1 and VEGF concentrations were analyzed. Furthermore the possible influence factors of HDAC1 protein expression level in the patients with lung adenocarcinoma were analyzed. Results The HDAC1 levels in the control group and observation group were（329.56±23.83）ng/L and（568.20 ± 35.40） ng/L, the difference was statistically significant（t= 23. 576, P = 0. 000）. The VEGF levels in the control group and observation group were（40.26±9.82）ng/L and（296.56± 19.80）ng/L respec- tively, the difference was statistically significant （t = 31.154, P =0. 000）. The HDAC1 protein level had statistical difference among different genders, ages, clinical stages and smoking history, the HDAC1 protein level in male,age 60 years old, clinical stage Ⅲ, and patients with smoking history were higher（P〈0.05）. The Pearson correlation analysis results showed that serum HDAC1 in the patients with lung adenocarcinoma was positively correlated with VEGF protein concentration（r= 0. 526, P= 0. 000）. The Logistic regression analysis showed that influence factor of HDAC1 protein expression level in the patients with lung adenocarcino- ma was clinical stage. Conclusion The high expression of HDACl protein in lung adenocarcinoma patients may also simultaneously regulate the VEGF expression,thus promotes the development of lung adenocarcinoma.