厚朴对D-半乳糖致脑老化小鼠学习记忆能力的影响

Effect of Cortex Magnoliae Officinalis on Learning and Memory

目的:探讨厚朴(Cortex magnoliae officinalis)对D-半乳糖所致脑老化小鼠学习记忆能力的影响及机制。方法:采用连续给小鼠皮下注射D-半乳糖方法,建立脑老化动物模型,用被动回避实验,Morris水迷宫实验检测小鼠学习记忆能力,同时测定小鼠脑组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)含量。结果:(1)被动回避实验:脑老化组的潜伏期显著短于对照组(P<0.01);错误次数显著多于对照组(P<0.01);而厚朴低、中、高剂量组的潜伏期显著长于脑老化组(P<0.05,P<0.01),中、高剂量组的错误次数显著少于脑老化组(P<0.01)。(2)水迷宫实验:脑老化组小鼠的逃避潜伏期显著长于对照组(P<0.05),而RAM低、中、高组的逃避潜伏期显著短于脑老化组(P<0.05,P<0.01)。(3)脑老化组脑组织SOD、GSH-Px活性显著降低,MDA含量明显增加;而厚朴可显著增加小鼠脑组织SOD、GSH-Px活性,降低MDA含量(P<0.01,P<0.05)。结论:厚朴可显著改善脑老化小鼠的学习记忆能力,其机制可能与抗氧化有关。

Objective：To investigate the effects of cortex magnoliae officinalis on learning-memory in D-galactose-induced brain aging mice.Methods：The brain aging model was induced by S.C D-galactose.Use passive avoidance test and Morris water maze to test learning-memory ability, and tested the activities of superoxide dismutase（SOD）,glutathione peroxidase （GSH-Px） in brain tissue of brain aging mice, and the content of malondialdehyde （MDA） were determined.Results：（1） Passive avoidance test： the latency in brain aging group significantly decreased and the number of errors significantly increased compared with the control group;number of errors （P〈0.01）.The latency low dose,middle dose and high dose cortex magnoliae officinalis group significantly increased,and the number of errors in middle dose and high dose RAM group significantly decreased compared with brain aging group （P〈0.05,P〈0.01）.（2）Morris water maze test： In brain aging group,the escape latency was significantly prolonged（P〈0.05）.The escape latency in 3 cortex magnoliae officinalis group was significantly shorter （P〈0.05,P〈0.01）.（3）In brain aging group the activities of SOD,GSH-Px and the content of MDA increase was significantly,cortex magnoliae officinalis can significantly improve activities of SOD,GSH-Px and reduce the content of MDA.Conclusion：Cortex magnoliae officinalis could significantly improve the ability of learning and memory in brain aging mice.The mechanism related to improve activities of SOD,GSH-Px and reduce the content of MDA in hippocampus.