摘要
[Objectives] This study was conducted to investigate the genetic diversity of 20 lotus( Nelumbo nucifera Gaertn) samples. [Methods]On this optimal ISSR amplification system,16 primers were screened with good polymorphism,and the DNA was used to amplify the 20 plant samples. [Results] The 16 primers produced 225 loci,of which 170 were polymorphic,and the polymorphic loci percentage was up to 75. 56%. The genetic similarity coefficients between the 20 varieties ranged from 0. 577 8 to 0. 951 1,which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups,the first of which included Baiyangdian red lotus and Donggua lotus,and other varieties was included in the second group. [Conclusions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.
[ Objectives ] This study was conducted to investigate the genetic diversity of 20 lotus （Nelumbo nucifera Gaertn） samples. [ Methods ] On this optimal ISSR amplification system, 16 primers were screened with good polymorphism, and the DNA was used to amplify the 20 plant samples. [ R^ults] The 16 primers produced 225 loci, of which 170 were polymorphic, and the polymorphic loci percentage was up to 75.56%. The genetic similarity coefficients between the 20 vari- eties ranged from 0. 577 8 to 0.951 1, which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups, the first of which included Baiyangdian red lotus and Donggua lotus, and other varieties was included in the second group. [ Conchmions] ISSR molecular markers could be effectively used in genetic diversity and fingerprint analysis for different lotus varieties.
基金
Supported by the Education Department Project of Fujian Province(JB11039)
