摘要
During an outbreak of diseases caused by co-infection with both parasites and bacteria in silver crucian carp （ Carassius auratus gibelio）, aerobic anoxy- genic phototrophic （AAP） bacteria in the aquatic environment were identified by PCR using the universal primers of bacterial 16S rDNA. The bacterial populations in the ponds infected and non-infected by the diseases were measured with acridine orange direct count （AODC） method, and the data were analyzed using SPSS software. The results showed that nine dominant cuhurable bacterial species： Shewanella putrefaciens, Acinetobacter sp. , Aeromonas sp. , Proteus vulgaris, Xan- thomonas sp. , Citrobacterfieundii, Morganella morganii, Vagococcus lutrae and Providencia sp. were identified, some of which were common pathogens. The bac- terial population in infected ponds was 7. 462 x 10^6 ind./ml in July, and 1. 007 4 x 10^7 ind./ml in August, and that in non-infected ponds was 1. 460 x 10x6ind./ml in July, and 1. 911 x10^6 ind./ml in August, with significant differences between the infected and non-infected ponds. The results suggested that the outbreak of diseases in silver crucian carp was to some extent related to the bacterial population in water environment.
During an outbreak of diseases caused by co-infection with both parasites and bacteria in silver crucian carp （ Carassius auratus gibelio）, aerobic anoxy- genic phototrophic （AAP） bacteria in the aquatic environment were identified by PCR using the universal primers of bacterial 16S rDNA. The bacterial populations in the ponds infected and non-infected by the diseases were measured with acridine orange direct count （AODC） method, and the data were analyzed using SPSS software. The results showed that nine dominant cuhurable bacterial species： Shewanella putrefaciens, Acinetobacter sp. , Aeromonas sp. , Proteus vulgaris, Xan- thomonas sp. , Citrobacterfieundii, Morganella morganii, Vagococcus lutrae and Providencia sp. were identified, some of which were common pathogens. The bac- terial population in infected ponds was 7. 462 x 10^6 ind./ml in July, and 1. 007 4 x 10^7 ind./ml in August, and that in non-infected ponds was 1. 460 x 10x6ind./ml in July, and 1. 911 x10^6 ind./ml in August, with significant differences between the infected and non-infected ponds. The results suggested that the outbreak of diseases in silver crucian carp was to some extent related to the bacterial population in water environment.
