HPLC法同时测定通脉颗粒中7种成分的含量

Simultaneous Determination of Seven Constituents in Tongmai Granule by HPLC

目的:建立同时测定通脉颗粒中丹参素、原儿茶酸、葛根素、大豆苷、阿魏酸、射干苷、丹酚酸B含量的方法。方法:采用高效液相色谱法测定。采用Agilent Zorbax SB-Aq色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸,梯度洗脱,流速1.2ml/min,柱温25℃。检测波长:丹参素、葛根素、大豆苷、丹酚酸B为287nm;原儿茶酸、射干苷为260nm;阿魏酸为322nm。结果:丹参素、原儿茶酸、葛根素、大豆苷、阿魏酸、射干苷、丹酚酸B的质量浓度分别在3.539~177.0μg/ml、0.3392~16.96μg/ml、2.755~137.8μg/ml、3.723~186.2μg/ml、1.699~84.96μg/ml、0.9440~47.20μg/ml、3.282~164.1μg/ml内与色谱峰峰面积呈良好的线性关系,平均回收率在92.4%~98.9%。结论:本方法简便、快速、可靠,可用于通脉颗粒的质量控制。

Objective： To establish method for the simultaneous determination of danshensu, protocatechuic acid, puerarin, daidzin, ferulic acid, belamcandin and Salvianolic acid B in Tongmai granules. Mthods ： Samples were determined by HPLC on Agilent＇ s column ZORBAX SB - Aq （4.6 × 250mm,5 pan）, which maintained at 25 ℃ column temperature, using 0. 1% （v/v） phosphoric acid - acetonitrile in a gradient elution mode as the mobile phase while the flow rate was 1.2ml/min. The detection wavelength were set at 287 nm for danshensu, puerarin, daidzin and Salvianolic acid B, 26Ohm for protocatechuic acid and belamcandin, 322nm for ferulic acid. Results： The linear ranges of danshensu, protocatecbuic acid, puerarin, daidzin, ferulic acid, belamcandin and Salvianolic acid B were 3. 539 - 177.0μg/ml,0. 3392 - 16. 96 μg/ml,2. 755 - 137. 8 μg/ml,3.723 - 186. 2 μg/ml, 1. 699 -84. 96 μg/ml,0. 9440 -47. 20 μg/ml,3. 282 - 164. 1 μg/ml, respectively. The average recoveries between 92. 4% - 98.9%. Conclusion： The method is simple, rapid and reliable, and it can be used for the quality control of Tongmai granules.