HPLC-RID测定银蓝通络口服液中银杏内酯含量

Determination of Ginkgolides in Yinlan Tongluo Oral Liquid by HPLC-RID

建立了高效液相色谱法测定银蓝通络口服液中银杏内酯的方法。采用酸性氧化铝柱处理银蓝通络口服液样品,用示差折光检测器,采用大连依力特Hypersil ODS2柱(150mm×4.6mm,5μm),流动相为甲醇∶水=28∶72(体积比),流速0.8mL/min,柱温35℃条件下,白果内酯(BB)、银杏内酯A(GA)、银杏内酯B(GB)和银杏内酯C(GC)分别在21.46~107.30、17.94~89.70、8.00~40.00、11.02~55.10mg/L内呈良好的线性关系,回归方程分别为Y=183.84 X-841.32(R^2=0.997 1)、Y=171.98 X-689.03(R^2=0.998 1)、Y=142.97 X-55.797(R^2=0.997 1)、Y=178.93 X-305.41(R^2=0.997 9),平均回收率分别为103.43%(RSD=1.49%)、100.86%(RSD=1.74%)、101.78%(RSD=1.39%)、102.49%(RSD=1.17%)。该法快速、简便、准确,重复性好,可用于银蓝通络口服液的质量控制。

To establish a HPLC-RID method for the determination of bilobide, ginkgolide A, ginkgolide B and ginkgolide C in the Yinlan Tongluo oral liquid.The chromatographic conditions included Hypersil ODS2 column（150 mm×4.6 mm,5 μm）, methanol-water（28 ： 72）at the flow rate 0.8 mL/min of the mobile phase, and at 35 ℃, RID. Respectively, the linear ranges of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were 21.46-107.30, 17.94-89.70, 8.00-40.00 and 11.02-55.10 mg/L. The linear regression equations of bilobide, ginkgolide A, ginkgolide B and ginkgolide C were Y=183.84X-841.32 （RZ=0.997 1）, Y=171.98X-689.03（R2=0.998 1）, Y=142.97X-55.797 （R2=0.997 1） and Y= 178.93X - 305.41 （R2 = 0. 997 9）, respecitively. The average recoveries of bilobide, ginkgolide A,ginkgolide B and ginkgolide C were 103.43% （RSD=1.49%）,100.86%（RSD= 1.74%）,101.78%（RSD= 1.39%） and 102.49% （RSD=1.17%）, respectively. The HPLC-RID method was precise, reproducible and stable, which can be applied in the quality control of Yinlan Tongluo oral liquid.