碳点与人血清白蛋白的相互作用研究

Interaction between Carbon Quantum Dots and Human Serum Albumin

以柠檬酸为原料制备了碳点,并运用紫外-可见分光光度计、荧光分光光度计和透射电镜(TEM)对该碳点进行光谱与形貌表征。采用荧光光谱、圆二色谱以及紫外-可见吸收光谱等方法,在近生理条件下研究了碳点与人血清白蛋白(HSA)的相互作用。荧光猝灭和紫外-可见吸收光谱试验结果表明,人血清白蛋白(HSA)和碳点形成了静态复合物,相互作用力以氢键和范德华力为主,而且碳点会引起人血清白蛋白(HSA)构象发生变化。研究结果可以为碳点的生物安全性研究提供一定的理论信息。

Carbon quantum dots （CQDs） was synthesized from citric acid. The structures and optical properties of the obtained CQDs were characterized by UV-vis absorption spectroscopy, photolumine- scence spectroscopy and transmission electron microscope（TEM）. The interaction between human serum albumin（HSA） and CQDs under physiological conditions was also investigated. The investigation was carried out with the use of fluorescence spectroscopy, circular dichroism （CD）spectra and UV-vis spectroscopy. The results of fluorescence quenching and UV-vis absorption spectra experiments indicated the formation of the complex of HSA and CQDs. The hydrogen bond and Van der Waals＇ force played predominant roles in the interaction process. Furthermore, it proved that CQDs could induce the conformational change of HSA. The research results provided a lot of important theoretic information for the biosafty evaluation of CQDs.