IGFBP7在甲状腺乳头状癌中的表达及意义

Expression of IGFBP7 in papillary thyroid carcinoma and clinical significance

目的观察胰岛素样生长结合蛋白-7(IGFBP7)在甲状腺乳头状癌中的表达及意义。方法对87例甲状腺乳头癌(PTC)组织及癌旁组织(PeT)进行IGFBP7、Ki67、p53免疫组织化学分析,其中10例提取蛋白用半定量western blot方法进行IGFBP7表达水平分析。结果 (1)IGFBP7免疫组化显示,PTC癌组织IGFBP7阳性率达78.16%(68/87),明显高于癌旁组织的31.03%(27/87)(P<0.05);(2)Western blot灰度值比较,癌组织IGFBP7表达水平显著高于癌旁组织(t=2.875,P<0.05);(3)Ki67、p53与IGFBP7相关性分析显示,p53表达水平与IGFBP7表达水平呈显著正相关性(r=0.261,P<0.05),而Ki67表达水平与IGFBP7表达水平无相关性(r=0.148,P=0.170);(4)高水平表达的IGFBP7与PTC淋巴结转移高度相关(r=0.238,P<0.05)。结论 IGFBP7表达异常增高可能与甲状腺乳头状癌发生及发展有关,有望成为诊断PTC的一项新型的生物标记物。

Objective To investigate the expression of insulin-like growth binding-protein-7（IGFBP7） for the patients with papillary thyroid carcinoma and analyze the clinical significance.Methods Immunohistochemistry method was used to analyze the expression of IGFBP7,Ki67 and p53 in 87 cases papillary thyroid carcinoma（PTC）tissues and peritumorial tissues（PeT）,and western blot was used to semi-quantitate the levels of IGFBP7 in 10 cases PTC and PeT.Results （1）The results of immunohistochemistry showed that expression of IGFBP7 in PTC tissues was obviously higher than that in PeT（78.16% vs 31.03%%）（P〈0.05）.（2）The consequences of western blot displayed that there was significant difference level of IGFBP7 between PTC and PeT（t=2.875,P〈0.05）.（3）The relative analysis of Ki67,p53 and IGFBP7 indicated that there was positive correlation between p53 and IGFBP7（r=0.261,P〈0.05）,nevertheless,there are no correlation between Ki67 and IGFBP7（r=0.148,P=0.170）.（4）High level of IGFBP7 is positively correlated with lymphatic metastasis prognosis（r=0.238,P〈0.05）.Conclusion Aberrant expression of IGFBP7 in papillary thyroid carcinoma maybe pathological mechanism of PTC,which as biomarker from biological pathway involving in the disease development and prognosis.