外周血NK细胞亚群检测在恶性血液病患者中的应用研究

Detection of peripheral blood NK cell subsets in patients with malignant hematological diseases

目的探讨外周血自然杀伤细胞(NK)亚群检测在恶性血液病患者中的应用价值。方法选择2014年3月至2016年4月于该院接受治疗的100例恶性血液病患者作为观察组,其中急性期50例,平缓期50例,并在同一时段内选择100例体检健康者作为对照组,比较各组间外周血NK细胞数及亚群的变化。结果急性期白血病及淋巴瘤患者NK细胞绝对数及相对数均远低于对照组,而平缓期白血病及淋巴瘤NK细胞绝对数及相对数高于急性期,差异具有统计学意义(P<0.05)。治疗后,白血病及淋巴瘤患者NK细胞绝对数及相对数均高于治疗前,差异具有统计学意义(P<0.05)。治疗前,白血病及淋巴瘤患者CD56^(bright)及CD56^(dim)水平与对照组比较差异有统计学意义(P<0.05);治疗后,两类患者CD56^(bright)及CD56^(dim)水平明显改善,治疗前比较差异有统计学意义(P<0.05)。结论恶性血液病患者外周血NK细胞数量降低,细胞功能受损。NK细胞亚群检测在评价恶性血液病患者预后及疗效方面有一定的指导意义。

Objective To study the clinical value of detection of peripheral blood natural killer （NK） cells subsets in patients with malignant hematological diseases. Methods A total of 100 patients with malignant blood disease were enrolled from March 2014 to April 2016 as the observation group, including 50 cases in acute stage and 50 cases in mild stage. At the same time, 100 health subjects were enrolled as the control group. The number of NK cells in peripheral blood and the changes of subgroups were compared. Results The number of NK cells in the different groups were compared, the absolute numbers and relative numbers of NK cells in the acute phase of leukemia and lymphoma were much lower than those in the control group（P〈0. 05）. While the abso-lute number and relative number of NK cells in mild stage of leukemia and lymphoma were higher than that in acute phase（P〈C 0. 05）. After treatment,the absolute number and relative number of NK cells in patients with leukemia and lymphoma were higher than before treatment（P〈0. 05）. Before treatment, levels of CD56bnght and CD56dim in patients with leukemia and lymphoma were statistically different with those of control group（P 〈0. 05）. After treatment, CD56bnght and CD56dim levels were significantly im-proved in both groups（P〈0. 05）. Conclusion The number of peripheral blood NK cells in patients with hematological malignancies could be reduced,and the cells function might be compromised. Detection of NK cell subsets could be with guiding significance in the treatment of malignant hematologic disease.