HPLC测定不同煎煮方法小柴胡汤中9种成分的含量

Different Decocting Methods Influence 9 Kinds of Ingredients of Xiao Chaihutang by HPLC

目的:考察去滓再煎法、不去滓煎法、两次煎法3种煎煮方法对小柴胡汤中9种成分含量的影响。方法:采用Welch Ultimate XB-C_(18)色谱柱(4.6 mm×150 mm,5μm),流动相乙腈-0.1%磷酸溶液,梯度洗脱,柱温26℃,流速1.0 m L·min^(-1),检测波长203,252,280 nm。结果:去滓再煎法中柴胡皂苷a的含量比不去滓煎法、现代两次煎法分别提高了62.41%,49.01%,柴胡皂苷d的含量比其他2种煎法分别提高了59.47%,28.71%,柴胡皂苷b1的含量比其他2种煎法分别提高了41.93%,16.14%。去滓再煎法中人参皂苷Re的含量比不去滓煎法提高了33.96%,比两次煎法提高了0.20%。去滓再煎法中黄芩苷,人参皂苷Rb1,甘草酸单铵盐,甘草苷4种成分的含量略低于两次煎法,高于不去滓煎法。不去滓煎法中各种成分的含量是3种煎法中最低的(6-姜辣素除外)。结论:该结果可有效反映不同煎煮方法的质量差异。在3种煎法中,去滓再煎的方法明显提高了柴胡在水煎液中有效成分的溶出,从一定程度上提升了小柴胡汤的临床功效,为张仲景《伤寒论》中对小柴胡汤采用去滓再煎的方法及质量评价提供了良好的实验依据。

Objective： To investigate the effect of deslagging and then decocting in Xiao Chaihutang. Method： HPLC was performed on Welch Ultimate XB-C18 column （4.6 mm × 150 mm, 5 μm） , with acetonitrile- 0.1% phosphoric acid solution as the mobile phase for gradient elution; the column temperature was 26 ℃ and flow rate was 1.0 mL.min-1 ; and the detection wavelength was set at 203, 252 and 280 nm. Result： The content of saikosaponin a in the decoction with deslagging and then decocting was 62.41% and 49.01% higher than the other two kinds of decoction; the content of saikosaponin d was 59.47% and 28.71% higher than the other two kinds of decoction; and the content of saikosaponin b1 was 41.93% and 16. 14% higher than the other two kinds of decoction. The content of Ginsenoside Re in the decoction with deslagging and then decocting was 33.96% and 0.20% higher than the other two kinds of decoction. In the method of deslagging and then decocting, the contents of baicalin, ginsenoside Rb1, ammonium glycyrrhetate and liquiritinwere slightly less than those in twice decocting method, but higher than those in no-deslagging and then decocting method. The contents of various constituents in no-deslagging and then decocting method was the lowest in three kinds of decoction method （except 6-gingerol） . Conclusion ： Results in this paper can effectively reflect the quality of the different decocting methods. In the three decocting methods, deslagging and then decocting method could significantly improve the effective components of bupleurum decoction in the dissolution, enhance the clinical efficacy of Xiao Chaihutang to a certain extent, and provide a good experiment basis for quality evaluation of deslagging and then decocting for Xiao Chaihutang from Shanghanlun.