反复熔铸对钴铬烤瓷合金细胞相容性的影响

Cytocompatibility of Co-Cr ceramic alloys after recasting

目的 :探讨反复熔铸对钴铬烤瓷合金生物相容性的影响。方法 :将钴铬烤瓷合金分别经过1~3次熔铸,使用DMEM细胞培养液进行浸提并采用电感耦合等离子体原子发射光谱仪(ICP-AES),检测浸提液中金属离子的种类和水平,使用合金浸提液培养小鼠肺结缔组织成纤维细胞(L-929),采用流式细胞仪检测L-929细胞周期的改变,Annexin-V-FITC/PI法检测L-929细胞凋亡与死亡情况。采用SPSS 13.0软件包对数据进行统计学分析。结果 :经过反复熔铸后,第3代合金与第1、第2代相比,除镓(Ga)的析出量增高外(P<0.05),其他金属离子的析出量及金属离子析出的总量均无显著变化(P>0.05);第1~3代合金对L-929细胞周期的影响无显著差异,早期凋亡率、晚期凋亡与坏死率差异均无显著性(P>0.05)。结论:经1~3次熔铸的钴铬烤瓷合金生物相容性未发生明显改变。

PURPOSE： To evaluate the cytocompatibility of Co-Cr ceramic alloys after recasting. METHODS： Cobalt- chromium （Co-Cr） ceramic alloys were recasted for 1-3 times. Samples of Co-Cr ceramic alloys were immersed in DMEM culture medium. The concentration of ions of leaching liquor was determined by ICP-MS total quantization method. L-929 cells were cultured with leaching liquor, flow cytometry was used to test cell cycle progression, Annexin-V-FITC/PI apoptosis staining kit was used to quantitatively detect cell death patterns. SPSS 13.0 software package was used for statistical analysis. RESULTS： After casting for 1-3 times, no significant increase in the concentration of ions of Co-Cr ceramic alloys was noted （P〉0.05） except Ga （P〈0.05） . No significant differences in L-929 cell cycle were found in Co- Cr ceramic alloys between the samples cast for 1 time and for 2-3 times （P〉0.05）. No significant difference in the rates of apoptosis and necrosis was found （P〉0.05）. CONCLUSIONS： The biocompatibility of Co-Cr ceramic alloys casted for 1 - 3 times is not changed.