桑枝多糖对免疫抑制小鼠的免疫调节作用

Immunoregulatory Functions of Mulberry Branch Polysaccharides on Immunosuppressed Mice

通过腹腔注射80 mg/kg环磷酰胺建立免疫抑制小鼠模型,给模型小鼠灌胃不同剂量的自制桑枝多糖,检测其免疫指标的变化情况。与正常对照组小鼠比较,模型对照组小鼠的脾脏指数、胸腺指数、腹腔巨噬细胞吞噬率与吞噬指数、淋巴细胞转化率、血清溶血素含量、溶血空斑形成数量均极显著降低(P<0.01);与模型对照组(灌胃等体积8.5 g/L Na Cl溶液)小鼠比较,桑枝多糖高剂量[600 mg/(kg·d)]给药组小鼠的胸腺指数和脾脏指数以及高剂量、中剂量[400 mg/(kg·d)]给药组小鼠的腹腔巨噬细胞吞噬率与吞噬指数、血清溶血素含量与溶血空斑形成数量均极显著提高(P<0.01),中剂量组小鼠的胸腺指数以及高、中剂量组小鼠的淋巴细胞转化率均显著提高(P<0.05)。试验结果表明,桑枝多糖能增强免疫抑制小鼠的免疫功能。

Immunosuppressed mice were established by intraperitoneal injection with 80 mg/kg cyclophosphamide. The model mice were then treated with various doses of self-made mulberry branch polysaccharides by intragastric administration to determine changes in immune indicators. The determined results showed that the index of thymus and spleen,phagocytic percentage and index of macrophages,transformation ability of lymphocytes,serum hemolysin content,and quantity of hemolytic plaque formation in model control group were significantly reduced（ P〈0. 01） compared with the normal control group. Compared with the model control group（ intragastric administration with the same volume of 8. 5 g/L Na Cl solution）,the index of thymus and spleen in high-dose mulberry branch polysaccharide group [600 mg/（ kg·d） ],the phagocytic percentage and index of macrophages,serum hemolysin content,and quantity of hemolytic plaque formation in high-and medium-dose group [400 mg/（ kg·d） ]were all increased at extremely significant level（ P〈0. 01）. Moreover,thymus index in medium-dose group and lymphocyte transformation rate in high-and medium-dose group were increased at significant level（ P〈0. 05）. These results indicate that mulberry branch polysaccharides can enhance immune function in immunosuppressed mice.